What is Alamar blue used for?
alamarBlue Cell Viability Reagent is an indigo-colored, non-toxic reagent that detects metabolically active cells and is used for the quantitative analysis of cell viability and proliferation.
What is Alamar Blue test?
alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. alamarBlue quantitatively measures proliferation in human, animal, bacterial, fungal, and mycobacterial cells.
What does MTS stand for in MTS assay?
2.6. Cell Proliferation Assay Cell proliferation was indirectly assessed with a colorimetric, (3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay obtained from Promega (Madison, WI, USA).
What is Alamar?
Alamar Blue Cell Viability Assay (6 Citations) AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent.
How does the Alamar blue assay measure cell viability?
AlamarBlue Cell Viability Reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction due to cell growth.
Is Alamar blue light sensitive?
alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature.
What MTS means?
MTS
| Acronym | Definition |
|---|---|
| MTS | Mountain Time Standard |
| MTS | Microsoft Technical Support |
| MTS | Mobile Telecommunications System |
| MTS | Message Transfer System |
Is MTS cytotoxic?
MTS assays are often used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. They are also used for the analysis of cytotoxic compounds like anticancer drugs and other toxic agents.
Why should I use XTT instead of MTT?
MTT is not a soluble product, so the cells must be lysed to solubilize the formazan salt before absorbance can be measured. XTT and resazurin do not require cell lysis, allowing kinetic monitoring of the same samples at different timepoints.
How do you make Blue Alamar?
All Answers (1)
- Dissolve alamar blue in PBS (pH 7.4) to 0.15 mg/ml.
- Filter-sterilize the resazurin solution through a 0.2 μm filter into a sterile, light protected container.
- Store the resazurin solution protected from light at 4°C for frequent use or at -20°C for long term storage.