What does atomic resolution mean?
Keywords: atomic resolution. The term `atomic resolution’ was defined a long time ago and it is generally accepted to correspond to an electron-density map (or a map calculated using another type of data, for example nuclear density) in which individual atoms can be distinguished.
What is the meaning of Resolution in crystallography?
Resolution is a measure of the quality of the data that has been collected on the crystal containing the protein or nucleic acid. Most crystallographic-defined structures of proteins fall in between these two extremes.
How is resolution determined in cryo-EM?
In cryo-electron microscopy, resolution is typically measured by the Fourier shell correlation (FSC), a three-dimensional extension of the Fourier ring correlation (FRC), which is also known as the spatial frequency correlation function.
How is resolution measured in cryo-EM?
In cryo-electron microscopy, resolution is typically measured by the Fourier shell correlation (FSC), a three-dimensional extension of the Fourier ring correlation (FRC), which is also known as the spatial frequency correlation function. To measure the FSC, the data needs to be separated into two groups.
What is good resolution for cryo-EM?
Since 2010, the average resolution of a cryo-EM structure has improved from 15 Å to about 6 Å, and it is increasingly common for cryo-EM to deliver protein structures in the range of 3–4 Å.
What is a crystallographic axis?
The crystallographic axes are imaginary lines that we can draw within the crystal lattice. These will define a coordinate system within the crystal. As we will see, the axes are defined based on the symmetry of the lattice and the crystal.
What do crystallographers do?
Crystallographers study diverse substances, from living cells to superconductors, from protein molecules to ceramics. Crystallography began with the study of crystals, like quartz. Today, crystallographers study the atomic architecture of any material that can form an orderly solid – from diamonds to viruses.