What are TSA cells?
TS/A cells express estrogen receptor and colony-stimulating factor and are negative for the adhesion protein ICAM-1 (2,3). The TS/A cell line is a well-characterized and highly published model for tumor metastasis and heterogeneity and is a popular tool for studies of immunological gene therapy (4,5).
What is TSA treatment?
Purpose: Trichostatin A (TSA), an antifungal antibiotic with cytostatic and differentiating properties in mammalian cell culture, is a potent and specific inhibitor of histone deacetylase (HDAC) activity.
Is Trichostatin a FDA approved?
To date, three HDAC inhibitors have been approved for cancer therapy by the US Food and Drug Administration (FDA). Vorinostat is structurally related to trichostatin A (TSA), a hydroxamic acid-containing natural product that was found to possess HDAC inhibitor activity and originally used as an antifungal drug.
What is deacetylation of chitosan?
The degree of deacetylation (DD, %) is defined as the molar fraction of GlcN in the copolymers (chitosan) composed of GlcNAc and GlcN [19]. For chitin, chitosan, and COS, measurement of the DD is crucial in determination of their chemical structures, physical properties, and interrelation.
What happens when a histone is deacetylation?
By deacetylating the histone tails, the DNA becomes more tightly wrapped around the histone cores, making it harder for transcription factors to bind to the DNA. This leads to decreased levels of gene expression and is known as gene silencing.
Is the nucleus Euchromatic vs heterochromatic?
The DNA in the nucleus exists in two forms that reflect the level of activity of the cell. Euchromatin is prevalent in cells that are active in the transcription of many of their genes while heterochromatin is most abundant in cells that are less active or not active.
What is Euchromatic and heterochromatic?
Definition. Heterochromatin and euchromatin are two major categories of chromatin higher order structure. Heterochromatin has condensed chromatin structure and is inactive for transcription, while euchromatin has loose chromatin structure and active for transcription.
Is chitosan an antifungal?
Chitosan, a product of chitin deacetylation, is considered to be potentially therapeutic for fungal infections because of its excellent biocompatibility, biodegradability and low toxicity. This study demonstrated that a potential antifungal mechanism of chitosan against C.
What is chitosan good for?
Chitosan is used for high blood pressure, high cholesterol, obesity, wound healing, and other conditions, but there is little scientific evidence to support many of its uses. In pharmaceutical manufacturing, chitosan is used as a filler in tablets, to improve the way certain drugs dissolve, and to mask bitter tastes.