What are the stain used in peripheral blood smears?
Commonly used stain in our environment is Leishman stain which is composed of polychrome methylene blue (basic component) and eosin (acidic component). May-Grunwald Giemsa or Wright-Giemsa stain can also be used.
How do you prepare for a peripheral smear?
Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin “feather” edge where all cells may be examined individually, particularly red blood cells.
Do you stain blood smears?
Left smear is unstained, right smear is stained with Wright-Giemsa stain. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.
What is a peripheral blood smear used for?
A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.
How do you stain PBF?
- Cover the blood smear with Leishman’s stain and keep it for 2 minutes.
- The stain should not be allowed to dry.
- Dilute with equal quantity of buffer solution (PH.6.8) or distilled water.
- Keep it for 8 minutes for the staining to take place.
- Then the slide is washed with distilled water.
What is the procedure for field stain?
Staining Procedure:
- Fill up two Coplin jars or wide-mouth bottles:
- Make blood smear on a clean glass slide and it is dried in the air.
- Fix in methanol for one minute or get Spray ‘Easyfix’.
- Dry in the air.
- Dip fixed smear to Field Stain B (Red Stain) for 5 to 6 seconds.
- Wash in running tap water.
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