What are different types of contamination in tissue culture?
Different biological contaminants causing contamination in plant tissue culture processes are:
- Bacteria.
- Yeast.
- Molds.
- Viruses.
- Mycoplasmas.
- Cross-contamination from other cell lines.
What are the bacteria commonly contaminate animal cell culture?
Bacterial species (Bacillus sp., Enterococcus sp. and Staphylococcus sp) are similarly some major contaminants of animal cell cultures.
What are the types of animal cell culture?
Types of animal cell culture
- 1.1 A. Primary cell culture. 1.1.1 Adherent cells. 1.1.2 Suspension cells. 1.1.3 Confluent culture and the necessity of sub-culture.
- 1.2 B. Secondary cell culture and cell line.
Which type of organism is the most likely contaminant in cell culture?
Mycoplasma is one of the most common cell culture contaminants, with six species of mycoplasma accounting for 95% of all contamination.
What is a contaminated culture?
A culture in which bacteria from a foreign source have infiltrated the growth medium.
What is cross contamination in animal cell culture?
The cross-contamination in a cell culture laboratory generally occurs due to failure in adopting good cell culture practices. Keeping more than one cell lines in a safety cabinet at the same time and using same tools when working with different cell lines.
What is contamination in cell culture?
Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, endotoxins, plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma, as well as cross contamination by other cell lines.
What are the four major instruments used in animal cell culture and write its uses?
What are the Basic equipments required for animal cell culture?
| Essential equipments | Beneficial equipments |
|---|---|
| Microscope | Cell counter |
| Sterilizer | Vacuum Pump |
| Washing up instrument | CO2 incubator |
| Sterilizing and drying oven | Preparation and quality control |
What is one of the limitation of animal tissue culture?
Limitations. It is not easy to control environmental factors like pH. Temperature, dissolved gases, etc.
What kind of contaminants can be found in cell culture?
Cell Culture Contamination. Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, endotoxins, plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma, as well as cross contamination by other cell lines.
How are animal cells affected by microbial contamination?
Culturing animal cells require a highly nutrient medium which attracts microorganisms and their spores, thereby exposing the cells to microbial contamination and compromising its growth and characteristics in culture medium.
Can a cell culture flask be contaminated with bacteria?
Although “cell culture contamination” typically conjures thoughts of bacteria among cells and cloudy media, unwanted invaders in the culture flask can take many forms.
How is cross contamination detected in cell culture?
DNA fingerprint, isotype analysis, karyotype analysis help to detect cross-contamination in cell lines,