Is immunoelectrophoresis quantitative or qualitative?
Immunoelectrophoresis (IEP) is an older method for qualitative analysis of M-proteins in serum and urine.
What is immunoelectrophoresis used for?
The method is mainly used clinically to determine the blood levels of immunoglobulins, and aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system and also in the diagnosis of multiple myeloma. A similar technique is called rocket immunoelectrophoresis.
Is immunoelectrophoresis quantitative?
Rocket immunoelectrophoresis is one-dimensional quantitative immunoelectrophoresis. The method has been used for quantitation of human serum proteins before automated methods became available.
How Rocket immunoelectrophoresis may be used quantitatively?
Rocket Immunoelectrophoresis is a quantitative method for serum proteins which involves electrophoresis of antigen into a gel containing antibody; the technique is restricted to detection of antigens that move to the positive pole on electrophoresis. It is a rapid way to quantify antigen in complex samples.
What is complement fixation technique?
The complement fixation test is a blood test in which a sample of serum is exposed to a particular antigen and complement in order to determine whether or not antibodies to that particular antigen are present. The nature of complement is to react in combination with antigen–antibody complexes.
What can immunoelectrophoresis diagnose?
What Abnormal Results Mean
- Multiple myeloma (a type of blood cancer)
- Chronic lymphocytic leukemia or Waldenström macroglobulinemia (types of white blood cell cancers)
- Amyloidosis (buildup of abnormal proteins in tissues and organs)
- Lymphoma (cancer of the lymph tissue)
- Kidney failure.
- Infection.
How immunoelectrophoresis is done?
Immunoelectrophoresis is a variation of the Ouchterlony double diffusion assay in gel technique, designed to analyze complex protein mixtures containing various antigens. Electrophoresis first separates proteins according to their size and mobility in the electric field within a gel matrix.
What is quantitative immunoelectrophoresis?
A quantitative irnmunoelectrophoresis method is described which in one electrophoretic run makes pos- sible. a simultaneous correlation of 15-20 immunologically related protein fractions in several different samples. Keywords: Serum proteins; immunoelectrophoresis, quantitative.