What is TuJ1 a marker for?
TuJ1 has been a marker of neurons in the central and peripheral nervous systems from the early stage of neural differentiation. FDCs expressed TuJ1 protein diffusely in both light and dark zones of germinal centers in all human lymphoid tissues. HK cells, cultured FDCs, also expressed TuJ1.
What is TuJ1 antibody?
The TUJ1 antibody reacts with beta-tubulin III, an ~50 – 55 kDa structural protein that is a component of tubulin. The beta III isoform of tubulin, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types.
What does TuJ1 do?
It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
What is beta-tubulin?
Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two of most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules.
What does NeuN stain for?
NeuN (neuronal nuclei) antibodies are fundamental tools for staining mature neurons and studying neuronal development and differentiation.
What are neural markers?
A neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells such as neurons. It allows detection and identification of cells by using different techniques. A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest.
Where is neurofilament located?
cytoplasm
Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. They are protein polymers measuring 10 nm in diameter and many micrometers in length.
Does MAP2 stain axons?
the caliber of the axon adjacent to the cell body is smaller than that of the dendrite, the angle between the primary neurite and the branch is smaller in dendrites compared to that in axons. Therefore, it is not necessary to stain for MAP2 (for a review of this topic see Cáceres et al, 2012).
What is alpha tubulin beta tubulin?
Alpha and beta tubulins are the building blocks of microtubules, one of the three components of the cell cytoskeleton. As compared to the other proteins (microfilaments and intermediate filaments) that make up the cell cytoskeleton, microtubules are larger (in diameter).
What is anti NeuN?
NeuN is a reported synonym of the human protein ‘RNA binding fox-1 homolog 3’, encoded by the gene RBFOX3. The full protein is reported to be 312 amino acid residues in length. The NeuN antigen is a documented neuronal marker.
What is NeuN a marker of?
Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in early human fetal nervous system.
Is the TUJ 1 antibody a neural stem cell marker?
Tuj 1, Neuron-specific class III beta-tubulin or Tubulin beta 4 Antibody Mouse monoclonal Tuj 1 (Neuron-specific class III beta-tubulin) is an excellent Neural Stem Cell Marker. Recognized as a rock solid antibody by our Customers. This Tuj-1 is referenced in publications with increasing frequency.
What kind of staining is used in tuj-1?
Tuj-1 staining of Neuron-specific class III β-tubulin in differentiated human neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 (green) secondary antibody (Molecular Probe, A-11001) and counterstained with PI (red).
How does TuJ1 antibody react with beta tubulin?
The TUJ1 antibody reacts with beta-tubulin III, an ~50 – 55 kDa structural protein that is a component of tubulin. Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha and beta tubulin subunits. The beta III isoform of tubulin, also known as neuron-specific class III…