What is the purpose of antibody microarray?
Antibody microarrays are often used for detecting protein expression from various biofluids including serum, plasma and cell or tissue lysates. Antibody arrays may be used for both basic research and medical and diagnostic applications.
What is antigen microarray?
Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
How do protein microarrays work?
In this technique, a library of antibodies, aptamers or affibodies is arrayed on the support surface. These are used as capture molecules since each binds specifically to a particular protein. The array is probed with a complex protein solution such as a cell lysate.
Is a beta-2 transferrin test accurate?
Conclusions The β2-transferrin assay is a reliable method for confirming suspected CSF and should be used as a primary screening method in all patients with suspected CSF leakage.
What is Beta-2 microglobulin blood test?
What is a beta-2 microglobulin tumor marker test? This test measures the amount of a protein called beta-2 microglobulin (B2M) in the blood, urine, or cerebrospinal fluid (CSF). B2M is a type of tumor marker. Tumor markers are substances made by cancer cells or by normal cells in response to cancer in the body.
How is CSF rhinorrhea treated?
Although CSF rhinorrhea has been traditionally treated with open surgical procedures, the introduction of the rigid endoscope for minimally invasive endonasal procedures has revolutionized treatment for many patients.
What is BAC microarray?
A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for transforming and cloning in bacteria, usually E. BACs are often used to sequence the genome of organisms in genome projects, for example the Human Genome Project.
What can beta trace protein test be used for?
Beta-trace protein test: new guidelines for the reliable diagnosis of cerebrospinal fluid fistula Quantitative measurement of betaTP is a noninvasive, highly sensitive, quick, and inexpensive method that can be used for the detection of CSF rhinorrhea in nasal secretions.
How to test for betatp in the blood?
Methods: Nephelometric detection of betaTP has been made in nasal secretion, serum, and CSF samples from healthy individuals as well as patients with reduced glomerular filtration rate and with bacterial meningitis. Additionally, 53 patients with suspected CSF rhinorrhea are also analyzed.
Why is it important to use an antibody array?
Antibody arrays allow a much broader view of protein activity than can be obtained with single-target ELISAs and Western blots. Moreover, antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.
Which is better beta2 transferrin or betatp?
Results: The betaTP test can also be used to reliably diagnose CSF rhinorrhea even slightly better than the beta2-transferrin test. It should not be used for patients with renal insufficiency and bacterial meningitis as they substantially increase serum and decrease CSF betaTP values, respectively.