What is the molarity of 37% formaldehyde?
12.7
| Name | Concentration (w/v %) | Molar concentration |
|---|---|---|
| Formaldehyde (CH2O) | 37 | 12.7 |
| Hydrochloric acid (HCl) | 37 | 12 |
| beta-mercaptoethanol (HSCH2CH2OH) | 100 | 14.2 |
| Phosphoric acid (H3PO4) | 85 | 14.7 |
How do you find molar concentration from percent concentration?
To convert from % solution to molarity, multiply the % solution by 10 to express the percent solution grams/L, then divide by the formula weight.
How do you calculate molar concentration of an enzyme?
The simple formula is: ( µg/mL ) = ( µM ) * ( MW in KD) , ( ng/mL ) = ( nM ) * ( MW in KD) , ( pg/mL ) = ( pM ) * ( MW in KD) . For example: If the protein molar concentration is labeled as 2 µM, and the MW of the protein is 40 KD, then this protein product’s mass concentration will be 2 ( µM ) * 40 ( KD ) = 80 µg/mL.
How do you make 37 formaldehyde from paraformaldehyde?
METHOD
- Working under a fume hood, in a glass scintillation vial, mix 0.92 g of paraformaldehyde in 2.5 mL of H2O and 35 μL of 1N KOH.
- Dissolve by heating on a hot plate while stirring with a magnetic stir bar.
- Cool the solution on ice.
- Filter through a 0.45-μm syringe filter.
What is the molar mass of formaldehyde?
30.031 g/mol
Formaldehyde/Molar mass
How do you convert 37 HCl to molarity?
Conversion % HCl to M HCl Thus, fuming/concentrated HCl 37% is 12 molar (= M = mol/L).
How do you calculate enzyme concentration from Vmax?
Vmax is equal to the product of the catalyst rate constant (kcat) and the concentration of the enzyme. The Michaelis-Menten equation can then be rewritten as V= Kcat [Enzyme] [S] / (Km + [S]). Kcat is equal to K2, and it measures the number of substrate molecules “turned over” by enzyme per second.
How would you determine the concentration of enzyme in an enzyme assay sample?
Enzyme assay
- Enzyme assays are laboratory methods for measuring enzymatic activity.
- The quantity or concentration of an enzyme can be expressed in molar amounts, as with any other chemical, or in terms of activity in enzyme units.
- Enzyme activity = moles of substrate converted per unit time = rate × reaction volume.
How big is the concentration of 2-mercaptoethanol?
Since the final concentration is 0.1 mM, and the pure solutions of 2-ME are 14.3 M, it is necessary to start ith a stock solution. Several companies sell diluted solutions of 2-ME; the 55 mM solution in PBS (Invitrogen catalog no. 21985-023) is a convenient concentration for a stock.
Do you need the BME molarity for mercaptoethanol?
I’m writing up a practical and $\\beta$-mercaptoethanol was used, with no concentration given. I’ve found I don’t need the BME molarity since I’m giving percentage (v/v) concentration, however I’m slightly confused as to why there are different values for its concentration.
Can a monothioacetal be prepared from 2-mercaptoethanol?
Monothioacetals are commonly prepared from 2-mercaptoethanol using strongly acidic conditions. Various catalysts are reported that avoid these harsh conditions. These catalysts are used with 2-mercaptoethanol or propanol and are highly selective for aldehydes over ketones.
How is GIBCO 2-mercaptoethanol used in cell culture?
Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration…