What is the meaning of HEp-2 cell line?
For example, the Hep-2 cell line, first described in 1954 as laryngeal cancer cells, was reported as soon as in 1966 to be comprised of cervical adenocarcinoma cells derived via HeLa cell line contamination.
What is a HEp-2 slide?
MBL Bion ANA (HEp-2) ANTIGEN SUBSTRATE SLIDES are intended for use as an aid in the diagnosis of certain autoimmune diseases. Among the methodologies available to detect ANA’s are EIA, ELISA, Dot Blot and the Indirect Fluorescent Antibody (IFA) technique.
Where do HEp-2 cells come from?
The HEp-2 cell line originated from tumours produced after injection of rats with epidermoid carcinoma tissue.
What is HEp cells?
Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
What does a positive ANA by HEp-2 mean?
The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA.
What is ANA HEp-2 positive speckled pattern?
Speckled. ANA with speckled pattern is associated to several rheumatic diseases and autoimmune liver disease. Approximately 5% of a healthy population normal serum show weak unspecific patterns.
What is HEp-2 blood test?
Summary: The presence of antinuclear antibodies (ANA) indicates the possibility of autoimmunity and the indirect immunofluorescence (IIF) assay on HEp-2 cells is the standard blood test (ANA-HEp-2) used to detect ANA.
What does a positive ANA HEp-2 substrate mean?
In most cases, a positive ANA test indicates that your immune system has launched a misdirected attack on your own tissue — in other words, an autoimmune reaction. But some people have positive ANA tests even when they’re healthy.
What does a positive ANA HEp-2 mean?
What does dual pattern ANA mean?
Results. A dual or mixed pattern may indicate disease overlap.