What is the fastest way to Destain coomassie gel?
FAST DESTAINING OF COOMASSIE GELS. Extract the gel from the sealing system (BioRad) alter running and introduce into a little tupperware with aprox 50ml of Coomassie solution (AcH : MeOH : H2O 10:45:45 with 0.25% Brilliant Blue G-250). Shake at RT and change de destaining solution until the bands can be observed.
How do you stain a coomassie?
Stain: Dissolve 0.4g of Coomassie blue R350 in 200 mL of 40% (v/v) methanol in water with stirring as needed. Filter the solution to remove any insoluble material. Add 200mL of 20% (v/v) acetic acid in water. The final concentration is 0.1% (w/v) Coomassie blue R350, 20% (v/v) methanol, and 10% (v/v) acetic acid.
Why do you microwave the gel with wash solution before doing the staining?
To speed up the procedure, heating the staining solution in the microwave oven for a short time is frequently used. By heating the gel during the washing and staining steps, the process can be finished faster and no toxic or hazardous compunds are evaporating.
How do you get rid of coomassie stains?
The blue-colored Kimwipes did not release the stain even when squeezed dry after incubation in various salts, water, or acid solutions for five weeks. The CBB removed thus can be simply disposed as solid waste and will not leach out from solid landfills.
Can you use Coomassie blue staining in the microwave?
After electrophoresis, incubate 1 or 2 gels in a staining container containing 100 ml Coomassie Blue R-250 staining solution. Caution: Use caution while performing the following steps using a microwave oven. Do not overheat the staining solutions. Loosely cover the staining container and heat in a microwave oven at full power for 1 minute.
How to make a Coomassie stain on glass?
Directions: 1) Remove SDS-PAGE gel from glass and rinse once in ddH2O in a suitable container with a lid. Try not to use a container much larger or much smaller then the gel. 2) Add enough Coomassie Stain to cover the gel by 1/2 inch (~ 1.5 cm). 3) Microwave on high power for 40 seconds to 1 minute (until the Coomassie Stain boils). 4)
How long do you incubate a Coomassie stain?
Add enough Coomassie Stain to cover the gel by 1/2 inch (~ 1.5 cm). 3) Microwave on high power for 40 seconds to 1 minute (until the Coomassie Stain boils). 4) Incubate the gel in the Coomassie stain for 5 to 10 minutes on a rocking table. If you did not microwave the Coomassie/gel, incubate for at least 1 hour.
What’s the best way to stain a coomassi gel?
Coomassie® R-250 Staining Protocol. Remove the staining container from the microwave oven and gently shake the gel for 15 minutes at room temperature on an orbital shaker. Decant the stain and rinse the gel once with deionized water. Prepare a destain solution containing 10% ethanol and 7.5% acetic acid.