What is P1 gene?
P1 genes expressed in the lytic pathway are those involved in the timing of phage development, in replication (from a “lytic” origin different from oriR), in the formation of phage particles, including headful packaging, and finally in cell lysis to release the phage progeny.
Which type of vector is P1?
It is one type of vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells. P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s. It is capable of Cre-Lox recombination.
Is P1 a temperate phage?
Phage P1 is a temperate phage of the Myoviridae family, possessing an icosahedral head and a long, contractile tail. The phage devotes several genes to controlling replication and partitioning of the plasmid so that it is stably maintained at low copy number.
What is PAC biotechnology?
P1 derived Artificial Chromosomes (PAC) is a genome derived from Phage P1. P1 phage utilises PAC sites for efficient breaking and packaging of the genome and its efficient delivery in transfection stage.
Which phage is known as temperate phage?
P1 is a temperate bacteriophage (phage) that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle, the phage genome exists as a plasmid in the bacterium, unlike other phages (e.g., the lambda phage) that integrate into the host DNA.