What is GelRed in electrophoresis?
GelRed™ is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Why is GelRed safer?
A number of dyes have been marketed as being safe because they are not mutagenic in lab tests. GelRed® and GelGreen® were designed specifically to be cell membrane impermeable, and therefore non-toxic and non-mutagenic. They are classified as non-hazardous waste under California Title 22.
What is Red safe used for?
RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA.
Is GelRed carcinogenic?
GelRedTM is a highly sensitive fluorescent nucleic acid dye that works when excited by a transilluminator. It was designed to replace EtBr, which is considered to be carcinogenic and mutagenic. GelRedTM is also known to be very sensitive without requiring a destaining step.
How do you use red safely?
Prepare a 100 ml of agarose gel solution (concentration from 0.8~3 %) in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat in until the solution is completely clear (about 2~3 minutes). Note : The thickness of gel should be less than 0.5 cm since thick gels may decrease sensitivity.
Is GelRed a carcinogen?
Is it safe to use GelRed and GelGreen?
The Ames test confirmed that GelRed® and GelGreen® are non-mutagenic at concentrations well above their working concentrations used for gel staining. Furthermore, environmental safety tests showed that GelRed® and GelGreen® are nonhazardous and non-toxic to aquatic life.
Which is more sensitive GelRed or GelGreen stain?
GelRed® and GelGreen® are highly sensitive either as precast gel stains or post gel stains. GelRed® is much more sensitive than EtBr, and unlike SYBR® Gold, GelRed® can also be used as a highly sensitive precast gel stain.
Which is more sensitive GelGreen or SYBR gold?
Unlike SYBR® Gold, GelRed® can also be used as a highly sensitive precast gel stain, and is available as a prestain loading buffer. GelGreen® is spectrally similar to SYBR® Safe but is far more sensitive, and can be used with blue light gel imaging systems like the DarkReader®.
Is the GelRed gel stain a registered trademark?
GelRed® and its uses are covered by granted and/or pending US and International patents. GelRed and EvaGreen are registered trademarks of Biotium, Inc. SafeView is a trademark of Applied Biological Materials; RedSafe is a trademark of iNtRON Biotechnology.