What is Alpha Minimum Essential Medium?
α-MEM (Minimum Essential Medium Eagle – alpha modification) is a medium based on MEM published in 1971 by Clifford P. Stanners and colleagues. It contains more non-essential amino acids, sodium pyruvate, and vitamins (ascorbic acid (vitamin C), biotin, and cyanocobalamin) compared with MEM.
Why use Alpha MEM?
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
What is Alpha Minimum?
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Balanced Salts, commonly referred to as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
Is Eagle’s MEM the same as MEM?
Gibco Minimum Essential Media (MEM) is patterned after Eagle’s Media and is well suited for the growth of a broad spectrum of mammalian cells.
What is the difference between alpha MEM and DMEM?
Background: Alpha modified Eagle’s minimum essential medium (α-MEM) and Dulbecco’s modified Eagle’s medium (DMEM) are the basic media for cell culture. α-MEM has minimum glucose and amino acid concentration than DMEM. Conclusion: α-MEM was superior to DMEM for in-vitro generation of ADMSC.
What is in RPMI media?
RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors.
What is DMEM used for?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is RPMI used for?
RPMI is used when laboratory testing requires fresh, unfixed specimens such as flow cytometry as well as some FISH, molecular and cytogenetics tests. 2 ) Why is it pink/red? It has a phenol red pH indicator that will turn from pink to yellow indicating contamination or exposure to air.
Is EMEM the same as MEM?
MEM Spinner was developed when calcium component was removed from the MEM medium, and resulted in a medium that supports the growth of cells in suspension. Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.
Is MEM and EMEM the same?
What is the purpose of Mem alpha modification?
MEM Alpha is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM Alpha Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
What is Minimum Essential Medium ( MEM ) used for?
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.
Who is the inventor of the minimum essential medium?
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
When do you use Minimum Essential Medium Eagle?
Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast. Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.