What happened in AP lab 13?
In this lab we found that the boiling test tubes remained clear throughout the entire time interval. The room temperature test tubes had the fastest rate, reaching the number four on the color chart. By existing knowledge it is a known fact that high temperature environments denature proteins and their active sites.
Why was the color indicator guaiacol used?
In this experiment an indicator for oxygen will be used. The compound guaiacol has a high affinity for oxygen, and in solution, it binds instantly with oxygen to form tetraguaiacol, which is brownish in color. Using a color palette is a relative way to compare a change and is therefore qualitative.
Is Tetraguaiacol a substrate?
This enzyme catalyzes the conversion of guaiacol (a colorless liquid substrate) and peroxide into tetraguaiacol (a brownish product) and water.
Was the limiting factor of your baseline reaction the enzyme or the substrate?
In the baseline date, the limiting factor was the enzyme since the more substrate produced, the more assembling of products required; however, there were limits to enzymes, for various factors were put to test out how the enzymatic activty would react according to the differentiating inserting of factors.
Is bromelain catabolic or anabolic?
CATABOLIC AND ANABOLIC ENZYMES Catalytic enzymes, called proteases, break down proteins and are found in many organisms; one example is bromelain, which comes from pineapple and can break down gelatin.
What color is oxygen in Guaiacol?
The compound guaiacol has a high affinity for oxygen, and in solution, it binds instantly with oxygen to form tetraguaiacol, which is brownish in color. The greater the amount of oxygen gas produced, the darker brown the solution will become.
Which of those factors do you think could affect enzyme activity?
Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.
What is the initial rate of this enzymatic reaction?
The initial rate of reaction is the gradient of the straight line portion of the plot, shown by the dotted red line. The initial rate of reaction is when concentrations of enzyme and substrate are known, so this allows fair comparison if you then change initial concentrations of enzymes or substrate.
What is the control in the enzyme lab?
The factors that remain constant are called the control variables. Control variables allow you to conclude that the results on enzyme activity obtained in your experiment are due to the varying of pH, the independent variable.