Is PEI toxic to cells?
PEI has a number of uses in laboratory biology, especially tissue culture, but is also toxic to cells if used in excess.
Is Polyethylenimine organic?
Polyethylenimine (PEI), an organic branched or linear polyamine polymer, has been successfully used in the past for DNA complexation and transfection in vitro and in vivo into several cell lines and tissues.
How is Polyethylenimine made?
Polyethylenimines (PEI) are low to high molecular weight compounds with the general formula -[CH2-CH2-NH2]-, made by ring opening polymerization of aziridine. These polymers are available as linear, partly branched or repetitively branched polymers (dendrimers).
Is PEI hydrophobic?
Poly(ethylenimine) (PEI) is an amino-rich highly hydrophilic cationic polyelectrolyte that has also been used as a functionalizing agent for carbon materials.
Is PEI a cationic?
Polyethylenimine (PEI) is a cationic polymer that contains repeating units composed of an amine group and a CH2CH2 spacer. Notably, PEI is an organic polymer that has the highest positive charge density potential as every third atom of this polymer is a protonable amino nitrogen.
What is PEI DNA?
Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery.
What is Polyethylenimine used for?
The synthetic polycation polyethylenimine is used to condense plasmid DNA into positively charged 100 nm complexes. These PEI–DNA complexes are then bound to adenovirus particles through charge interactions with negative domains on the viral hexon.
Is PEI conductive?
Conductive for electricity. Flame resistance (94-V-0 flammability rating) Good chemical resistance. Resistance to hot water and steam.
Is Polyethylenimine biodegradable?
Polyethylenimine (PEI) is a class of cationic polymers proven to be effective for gene delivery. However, PEI is nondegradable and the molecular weight of PEI affects the cytotoxicity and gene transfer activity.
Is PEI a surfactant?
The results indicate that the phase behaviour of PEI : cationic surfactant mixtures under dilute conditions can be tuned by altering head-group and counterion.
Is PEI biodegradable?
Why are quaternary ammonium Polyethyleneimine used in medical devices?
These findings confirm previous reports that polycations bearing quaternary ammonium moieties inhibit bacterial growth in vitro and have a potential use as additives in medical devices which need antibacterial properties. 1. Introduction During the last two decades continuous efforts have been made to develop polymers with antimicrobial properties.
How is Polyethyleneimine prepared in cationic polymerization?
Polyethyleneimine (PEI) is a synthetic polymer prepared from aziridine by cationic polymerization. Its structure contains primary, secondary, and tertiary amino groups due to chain transfer reactions. The amino group ratio is 1 : 2 : 1 (i.e., 25% of primary amines, 50% of secondary amines, and 25% of tertiary amines).
What makes QA-Pei an antibacterial reagent?
Activity analysis revealed that the degree of alkylation and N-methylation of the QA-PEI nanoparticles plays a significant role in antibacterial activity of the reagent. The most potent compound was octyl alkylated QA-PEI alkylated at 1 : 1 mole ratio (primary amine of PEI monomer units/alkylating agent).
What kind of bacteria are inhibited by QA Pei nanoparticles?
QA-PEI nanoparticles completely inhibited bacterial growth (>10 6 bacteria), including both Gram-positive, that is, Staphylococcus aureus at 80 g/mL, and Gram-negative, that is, Escherichia coli at 320 g/mL.