How do you clean a biosafety cabinet?
The cabinet must be completely cleared of any equipment, pipettes, waste, liquids and tubing. Decontaminate the surfaces of the BSC with an appropriate disinfectant, such as a 10% bleach solution followed by 70% ethanol solution.
What is BSC cleaning?
In-house cleaning operations and building service contractors (BSCs) often come out of their corners to defend their titles, and prove to those betting on them for best results that they are indeed the true champions of clean.
How should you handle a small spill in the biosafety cabinet?
Spills inside of a Biological Safety Cabinet (BSC)
- Leave BSC on.
- If the spill may involve an aerosol, instruct all occupants to leave the room for 30 minutes to allow aerosols to settle.
- Remove contaminated lab coat or clothing and wash exposed skin.
- Put on clean gloves and lab coat.
How do you clean up microbial spills?
Prepare enough volume of a 1:10 dilution of chlorine bleach or other approved disinfectant to saturate the contaminated area. Contain the spill with paper towels or other absorbent material such as bench liners. Flood the spill area with disinfectant. Allow 30 minutes of contact time.
Can you use bleach in biosafety cabinet?
Answer: One of the more archaic methods to decontaminate a biosafety cabinet is bleach. However, no lab should rely on it as a regular cleaning method. Bleach, while a germicide, is corrosive to the steel that most biosafety cabinets are made of. Therefore, you should limit your use of bleach to emergencies only.
How often should BSC be cleaned?
3. The BSC is cleaned daily prior to use and after each use. If the hood is not used, no cleaning needs to be performed.
What solution is used to clean biological spills?
bleach solution
General guide for ALL biological spills A fresh 10% household bleach solution is commonly used as a disinfectant; allow 20-30 minutes’ contact time (however, use the recommended disinfectant and contact time for the material you are handling).
What do all biosafety cabinets have in common?
There are 3 main classes of Biological safety cabinets (BSCs) – the thing they all have in common is that they protect the worker/environment from the cultures. Class II BSCs are the most common cabinets found in the lab and are the type of cabinet used for mammalian cell culture.
How do you remove sharps from a spill?
Clean up & dispose of the debris
- Use tongs, scraper, cardboard or dustpan to remove any sharps, place in sharps container.
- Remove the absorbent material by using a dust pan and deposit in a biohazard bag along with the dust pan.
- Spray the area with disinfectant and allow a further 10 minutes contact time.
How do you clean biological hazards?
Remove disinfectant with paper towels or other absorbent material and dispose of in biohazard bad. Wipe off any residual spilled material and reapply disinfectant before final clean up. Wipe equipment with equipment compatible disinfectant (e.g., non-corrosive). Rinse with water if necessary.