Does RPMI 1640 contain glucose?
One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
What is the difference between DMEM and RPMI?
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
How can DMEM make blood sugar high?
-For 1 lt 50 mM high glucose DMEM, you need 44.8 ml of 1 M glucose and 955.2 ml of 5.5 mM DMEM, if you wanna make a solution from a liquid glucose concentration. – Or else simply add 8 g of D-glucose into the 1 L of low glucose DMEM.
Can you use DMEM with high glucose levels?
DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
What is the DMEM in Basal Medium Eagle?
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells.
What makes DMEM unique from other essential media?
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
What kind of cells can be grown in DMEM?
Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.