What is ZsGreen1?
ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein (ZsGreen) that has been engineered for brighter fluorescence and higher expression in mammalian cells.
What is the difference between GFP and EGFP?
The key difference between GFP and EGFP is that the GFP is a wild-type protein incorporated in the molecular cloning of non-mammalian cells while the EGFP is an improved or engineered type of GFP that can be used on mammalian cells.
What does tdTomato mean?
tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifically designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1).
What is ZsGreen used for?
The expression of Zoanthus sp. green fluorescent protein (ZsGreen) in differentiated cells was readily detectable. This can be used as an ideal cell transplantation model to study the distribution of cells.
What is a tdTomato mice?
tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is as photostable as mCherry (Shaner et al. 2004).
Is mCherry far red?
Far-red fluorescent proteins are beneficial for imaging in mammals. Here, starting from mCherry, the most commonly used among the different types of red fluorescent proteins (RFP), not having a H-bond network in its original form, we sought to recover the hydrogen bond network in mCherry.
Why is green fluorescent protein important?
Biologists use GFP to study cells in embryos and fetuses during developmental processes. Biologists use GFP as a marker protein. If the cell produces the green fluorescence, scientists infer that the cell expresses the target gene as well. Moreover, scientists use GFP to label specific organelles, cells, tissues.