What is the principle behind the hematoxylin and eosin staining?
The principle behind H & E stain is the chemical attraction between tissue and dye. Hematoxylin, a basic dye imparts blue-purple contrast on basophilic structures, primarily those containing nucleic acid moeties such as chromtatin, ribosomes and cytoplasmic regions rich in RNA.
Why is hematoxylin used in staining?
Hematoxylin and eosin (H&E) is the most widely used stain in histology and allows localization of nuclei and extracellular proteins. Hematoxylin, not a dye itself, produces the blue Hematin via an oxidation reaction with nuclear histones causing nuclei to show blue.
What does hematoxylin stain reveal?
The H&E stain provides a comprehensive picture of the microanatomy of organs and tissues. Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers, muscle fibers and red blood cells.
How does hematoxylin eosin work?
H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors.
How does hematoxylin function as a cellular stain?
The hematoxylins and eosin Essentially, the hematoxylin component stains the cell nuclei blue-black, showing good intranuclear detail, while the eosin stains cell cytoplasm and most connective tissue fibers in varying shades and intensities of pink, orange, and red.
Why is hematoxylin basic?
Haemotoxylin is actually a dye called hematein (obtained from the log-wood tree) used in combination with aluminium ions (Al3+). It is used to stain acidic (or basophilic) structures a purplish blue. (Haematoxylin is not strictly a basic dye, but it is used with a ‘mordant’ that makes this stain act as a basic dye.
Why does haematoxylin require a mordant?
Haematoxylin was extracted and oxidised in boiling water to form hematein. Hematein is anionic with poor affinity for tissue. It requires the presence of a mordant to impart a positive charge to the complex thus enabling binding to anionic tissue components like nuclear chromatin.
What is the use of hematoxylin?
Hematoxylin and eosin (H&E) are common stains to examine the cells and tissues of a melanoma sample. They stain basophilic cellular structures blue (such as nuclei) and eosinophilic structures shades of red, pink, and orange.
What does iron hematoxylin stain?
intestinal protozoa
Iron hematoxylin was the stain used for most of the original morphological descriptions of intestinal protozoa found in humans. The iron hematoxylin staining method can stain the intracellular and nuclear structure’s dark compared to the cytoplasm, for easy identification of parasite morphologies.
How does trichrome staining work?
Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by tinting them in contrasting colours.