What is SuperScript 3?
Documents. FAQ. Citations & References. Additional Information. Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
What is the purpose of Ssiii?
The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.
How do you type 3?
Hold down the “Alt” key and type “0179” without quotes. When you release the “Alt” key, the cubed symbol appears. However, this Alt code isn’t universally supported, so if you’re getting strange results, try using the Character Map or superscript effect.
What is RT qPCR used for?
RT-qPCR is used in a variety of applications including gene expression analysis, RNAi validation, microarray validation, pathogen detection, genetic testing, and disease research.
What is Invitrogen used for?
Invitrogen currently offers more than 25,000 products and services to support research in cellular analysis, genomics, proteomics, and drug discovery, and seeks to address research problems in developing fields, including biodefense and environmental diagnostics, bioinformatics, epigenetics, and stem cell research.
How do you type a small 3?
Using the Alt Code Hold down the “Alt” key and type “0179” without quotes. When you release the “Alt” key, the cubed symbol appears. However, this Alt code isn’t universally supported, so if you’re getting strange results, try using the Character Map or superscript effect.
What can superscript III RT be used for?
Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
How much RNA can be detected with superscript III?
RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability.
How is superscript III used to synthesize cDNA?
The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
How big is a superscript III reverse transcriptase vial?
SuperScript™ III Reverse Transcriptase (4 × 10,000 units total, at 200U/µL) is supplied with a vial (4 × 1 mL) of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl 2 ], and a vial (4 × 500 µL) of 100 mM DTT. Store at -20°C. Convenient, on-site access to the products you need. Learn more.