What is meant by inoculum effect?
The inoculum effect (IE) is a laboratory phenomenon that is described as a significant increase in the minimal inhibitory concentration of an antibiotic when the number of organisms inoculated is increased. The IE generally occurs with beta-lactam antibiotics in relation to beta-lactamase-producing bacteria.
What does inoculation mean?
Definition of inoculation 1 : the act or process or an instance of inoculating especially : the introduction of a pathogen or antigen into a living organism to stimulate the production of antibodies. 2 : inoculum.
What is the meaning of inoculum in microbiology?
Thus, in microbiology, the term inoculum refers to the material in which microbiologists used for inoculation, i.e., the introduction of microorganisms into a culture medium. …
What does source of inoculum mean?
In biology, inoculum refers to the source material used for inoculation. In medicine, material that is the source of the inoculation in a vaccine. In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture.
What is inoculum in plant pathology?
Inoculation is the pathogen or any part of the pathogen that contacts with the plant at certain site to initiate the infection process, such as spores, sclerotia, or fragments of mycelium of fungi may be fungal inoculum.
Is the eagle effect real?
The Eagle effect, described by Harry Eagle in 1948, occurs when high concentrations of antibiotic above the MBC result in an increased proportion of surviving cells (Eagle, 1948). Bacterial cells exposed to higher concentrations of antibiotic exhibited a decreased rate of cell death in this study (Eagle, 1948).
What is the difference between vaccine and inoculation?
Of the three words, vaccinate is the most narrow because it specifically means to give a vaccine to someone. Inoculate is more general and can mean to implant a virus, as is done in vaccines, or even to implant a toxic or harmful microorganism into something as part of scientific research.
What is inoculum with example?
The definition of an inoculum is a substance introduced into the body to create or increase the body’s resistance or immunity to a disease. A flu vaccine is an example of an inoculum. The active material used in an inoculation; an inoculant.
What is an inoculum in microbiology quizlet?
What is an inoculum? The bacteria transferred to a new medium. The bacteria that are transferred during inoculation are called the inoculum. The new sterile medium receives, or is inoculated with, the inoculum.
How do you make an inoculum?
6.1. Inocula are prepared by growing C. neoformans in liquid YPAD overnight at 30 °C. Cells are counted by hemocytometer and, for an intranasal infection, 1×107 cells are washed twice with PBS and resuspended in 1 ml of PBS. Fifty microliters of this inoculum are used per mouse (5×105 cells).
What is inoculum and infection?
Inoculation is the initial contact of a pathogen with a site of plant where infection is possible. The pathogen(s) that lands on or is otherwise brought into contact with the plant is called the inoculum. The inoculum is any part of the pathogen that can initiate infection.