What is LR Clonase?
Gateway® LR Clonase® II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone.
What is a Clonase enzyme?
Gateway BP Clonase enzyme contains both Int (Integrase) and IHF (Integration Host Factor) proteins that catalyze the in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and a Donor vector (containing attP sites) to generate Entry clones.
What does BP Clonase do?
Gateway® BP Clonase® II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry clones.
How does LR Clonase work?
The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone with all of the components necessary for gene expression. This reaction is catalyzed by the LR Clonase enzyme mix. As a result, an expression clone with the DNA of interest flanked by attB sites is generated.
What is an entry clone?
An Entry clone contains your gene of interest flanked by attL sequences, which are then used to recombine with attR sequences to create your desired expression clone.
What is entry clone?
What is an attB site?
The attB site is a short DNA sequence (less than 30 bp) corresponding to the crossover region at which strand exchange takes place (7). Two imperfect inverted repeats that bind to the integrase surround a 7-bp overlap region delimited by the scattered cuts made by the recombinase.