What is Fluoromount-G?
Fluoromount-G Mounting Medium is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. It is water soluble and used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C.
How do you use Fluoromount-G?
Instructions For Use Remove one slide at a time from aqueous buffer, add one (1) drop of Fluoromount-GT directly to cell prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip. Allow mounted preps to air-dry for five (5) minutes before examination.
Does Fluoromount-G have glycerol?
Fluoromount-G ( Electron Microscopy Sciences ): phosphate buffered glycerol + 10% polyvinyl alcohol + 0.1% sodium azide.
How long does it take for Fluoromount to dry?
Allow Fluoromount to dry for 30–45 minutes at room temperature.
Does Fluoromount G Harden?
Fluoromount-G® Anti-Fade is a non-hardening, aqueous-based compound for mounting slides used in microscopy applications in which the preservation of fluorescence intensity is critical.
What are the types of mounting medium?
These media are of three types: The syrups, Gelatin media, and Gum Arabic media. Some of the metachromatic stains tend to diffuse from the sections into mounting media shortly after mounting: this may be prevented by using fructose syrup. Gome stains, e.g., methyl violent tend to diffuse into medium after mounting.
How do I get rid of Fluoromount?
Removal of Fluoromount Fluoromount is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water. Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.
How do you unmount slides?
Soak the slides in xylene until the coverslips are removed. Rinse off residual medium from the tissue sections in 2 changes of fresh xylene; followed by descending grades of alcohol starting from an absolute alcohol (2 changes) ending in 95% (1 change), followed by DI water.
How long does ProLong gold take to dry?
24 hours
Like the original ProLong® antifade reagent, ProLong® Gold reagent will cure within 24 hours, and the sample can be saved for months after mounting.
When to use fluoromount G for staining?
Fluoromount-G™ is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. This water-soluble Fluoromount-G™ is used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C.
What kind of mounting medium does fluoromount-G use?
It forms a semi-permanent seal for prolonged storage of slides at 2-8°C. Fluoromount-G Mounting Medium does not fluoresce and may reduce the amount of fluorochrome quenching and photobleaching during fluorescence microscopy. This medium is supplied as a 1X stock solution containing sodium azide.
What is the refractive index of fluoromount G?
This mounting medium also provides a semi-permanent seal for storage of slide preparations. The refractive index of Fluoromount-G® is reported to be ~1.4 70 .
What kind of preservative is in fluoromount GT?
Fluoromount-GT is supplied as a 25 ml solution containing 0.1% sodium azide as preservative; store at room temperature. Fluoromount™ is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water: