What is DRAQ5?
DRAQ5™ Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins.
What does DRAQ5 bind?
DRAQ5 was found to intercalate or bind to the minor groove of AT-rich regions [7,10]. In theoretical simulations, a bi-mechanistic interaction was also suggested [11]. We recently categorized DRAQ5 as a concentration dependent intercalator and a minor-groove binder [5].
Does DRAQ5 stain dead cells?
DRAQ5™ staining is accelerated at 37ºC and maybe reduced to 1-3 min. DRAQ5™ stains intact, live, fixed, permeabilized and dead cells. Cells can be analysed directly without further treatment or washing.
What is DAPI staining used for?
DAPI staining was used to determine the number of nuclei and to assess gross cell morphology. Following light microscopic analyses, the stained cells were processed for electron microscopy.
How is the DRAQ5 fluorescent probe used in cellular imaging?
DRAQ5™ Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. Because of its far-red excitation and emission, the DRAQ5 Stain can be multiplexed with many other fluorophores.
What kind of dye is used in DRAQ5?
DRAQ5 ®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells.
What kind of filters are used for DRAQ5?
It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters. DRAQ5™ can also be used in cell imaging and is a good replacement for DAPI, as it does not get excited by UV or Violet lasers and is sub-optimally excited by the 488 nm laser.
How is DRAQ5 used in cell cycle analysis?
DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells. It is optimally excited at 568 nm, 633 nm, and 647nm and can be detected using 695LP, 715LP, and 780LP filters.