What are some features that make zebrafish attractive for research?
because low cost and easy maintenance, transparent embryo, easy manipulation, high fecundity, and rapid embryonic development favor the zebrafish as an attractive model for in vivo assays with simplicity and versatility of in vitro assays over mammalian models which lack all of these benefits.
Why are zebrafish embryos used in research?
Zebrafish embryos are nearly transparent which allows researchers to easily examine the development of internal structures. As zebrafish eggs are fertilised and develop outside the mother’s body it is an ideal model organism for studying early development. Zebrafish have a similar genetic structure to humans.
How do zebrafish embryos grow?
For optimal growth during the first few days, keep the embryos in system water at least 3 cm deep (25-50 embryos per 100 ml in a 250 ml beaker, for example). Embryos can also be raised 25 per 35 mm petri dish if the water is changed daily, although this method does not provide for optimal growth.
What was the purpose of this experiment zebrafish?
In addition to utilizing zebrafish disease models to characterize human diseases, researchers can also identify and test new drugs to treat the diseases being modeled. The ability of zebrafish to generate many embryos every time they breed makes them especially useful for high throughput drug screening.
How are zebrafish used as animal models for research?
Because of its fully sequenced genome, easy genetic manipulation, high fecundity, external fertilization and rapid development, and nearly transparent embryo, zebrafish are a unique model animal for biomedical research, including studies of biological processes and human diseases.
How do the zebrafish cells divide?
As students come to understand the role mitosis plays in development, they will be confronted with the idea that all the cells in the zebrafish must have the same DNA. In multicellular organisms individual cells grow and then divide via a process called mitosis, thereby allowing the organism to grow.
What do zebrafish embryos eat?
Young larvae can be fed with dry food of ~100 microns in size (e.g., ZM100) or live food such as paramecium and rotifers (which stimulates growth). The food size can slowly be increased to 200 microns (e.g. ZM200) or 300/400 microns (e.g. ZM300). A population of adult fish should be around 6-7 fish per liter of water.
How long do zebrafish eggs take to hatch?
Laboratory zebrafish embryos and larva are normally maintained in incubators during the first week (7 days) after egg fertilization. Zebrafish embryos dechorionate (hatch) at approximately 72 hours post fertilization [3 days post fertilization (dpf)].
How many eggs do zebrafish lay?
After 3 – 4 months zebrafish are sexually mature and can generate new offspring. A single female can lay up to 200 eggs per week.
What are the parts of blastocyst?
The blastocyst is therefore comprised of an inner cell mass, which subsequently forms the embryo, and a surrounding outer layer of cells, the trophoblast, which later forms the outer chorionic sac and the fetal component of the placenta.
How are gene products added to zebrafish embryos?
One of the strengths of the zebrafish model system is the ease with which specific gene products can be added to or eliminated from the embryo by microinjection. To ubiquitously overexpress a particular protein, the mRNA encoding it is injected into the yolk at the 1-cell stage.
How is zebrafish used as a model system?
Zebrafish ( D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed.
What are the seven stages of zebrafish development?
ABSTRACT We describe a series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio. We define seven broad peri- ods of embryogenesis-the zygote, cleavage, blas- tula, gastrula, segmentation, pharyngula, and hatching periods.
How many embryos are injected with Morpholino per hour?
The vehicle for adding either mRNA or morpholino to an embryo is microinjection. Microinjection is efficient and rapid, allowing for the injection of hundreds of embryos per hour. This video shows all the steps involved in microinjection.