How does Strep-tag work?
The Strep-tag ®II binds specifically to the engineered streptavidins, Strep-Tactin ® and Strep-Tactin ®XT, by occupying the binding pocket of the natural ligand biotin. Hence, the interaction is easily reversible by excessive addition of the competitor.
Does anti strep antibody bind biotin?
Free biotin binds to Strep-Tactin ® and consequently inactivates the resins. It has to be removed or masked prior to affinity chromatography. For this purpose, we recommend our ready-to use Biotin Blocking Solution or avidin.
What does biotin as D biotin mean?
D-Biotin is the naturally occurring and biologically active form of Biotin. There are 7 other isomers (or versions) of Biotin, but this is the most active form and also the form used in the clinical studies that were conducted.
What binds to Strep-tag?
The tag binds to Strep•Tactin® protein, which is an engineered streptavidin with an optimized Strep•Tag® II peptide binding site. The Strep•Tag® II peptide binds to Strep•Tactin® protein nearly 100 times tighter than it binds to streptavidin, but elutes under gentle, physiological conditions.
Which is more binding affinity Strep tag or streptavidin?
The binding affinity of Strep-tag to Strep-Tactin is nearly 100 times higher than to Streptavidin. The so called Strep-tag system, consisting of Strep-tag and Strep-Tactin, has proven particularly useful for the functional isolation and analysis of protein complexes in proteome research.
Which is better Streptactin XT or Strep Tactin?
HABA is used for regeneration from Strep-Tactin® and in case of Strep-Tactin®XT 3 M MgCl2 is applied. Furthermore, higher protein yields compared to Strep-Tactin ® are obtained. On average, StrepTactin ® XT provides almost 2-fold more protein than Strep-Tactin ® .
How is Strep Tactin used for protein purification?
The highly specific interaction of the Strep-tag ® II with Strep-Tactin ® ensures efficient one-step purification of the protein of interest in unparalleled purity even from crude cell lysates.
How does the Strep tag 2 sequence work?
Strep•Tag II sequence is an 8-amino acid fusion tag with binding specificity comparable to biotin. The small size of the tag reduces potential interference with target protein structure or function. The tag binds to Strep•Tactin® protein, which is an engineered strepta-vidin with an optimized Strep•Tag II binding site. Strep•Tag II binds