How does Compound C inhibits AMPK?
Compound C inhibited AMPK kinase activity in a dose dependent manner in multiple glioma cells as observed by reduced phosphorylation of the canonical AMPK substrate ACC (Fig 1B). Importantly, the effect of Compound C on normal astrocytes was significantly less than that on glioma cells (Fig 1E).
How does Dorsomorphin work?
Dorsomorphin inhibits osteogenic differentiation of myofibroblasts in vitro and alters bone mineralization in zebrafish, which is consistent with inhibition of BMP signals critical for osteoblast commitment.
What are the BMP inhibitors?
Sclerostin, chordin, CTGF, follistatin, and gremlin are additional BMP antagonists that may act in the skeleton to regulate BMP availability. Another class of BMP inhibitors are the proteins that bind to BMP receptors but have no inherent signaling function and thus act as BMP receptor antagonists.
What is Aicar used for?
AICAR is an analog of adenosine monophosphate (AMP) that is capable of stimulating AMP-dependent protein kinase (AMPK) activity. AICAR has been used clinically to treat and protect against cardiac ischemic injury. The drug was first used in the 1980s as a method to preserve blood flow to the heart during surgery.
Is BMP a neural inducer?
The default model, based initially on Xenopus studies, has proposed that bone morphogenetic protein (BMP) inhibition is necessary and sufficient for neural induction (1).
Does BMP have potassium level?
The basic metabolic panel (BMP) is a blood test that gives doctors information about the body’s fluid balance, levels of electrolytes like sodium and potassium, and how well the kidneys are working.
Is AICAR illegal?
As of January 2011, AICAR was officially a banned substance in the World Anti Doping Code, and the standard levels in elite athletes have been determined, to interpret test results.
What is the molecular weight of dorsomorphin K I?
Molecular Weight(MW): 399.49. Dorsomorphin is a potent, reversible, selective AMPK inhibitor with K i of 109 nM in cell-free assays, exhibiting no significant inhibition of several structurally related kinases including ZAPK, SYK, PKCθ, PKA, and JAK3. Also inhibits type I BMP receptor activity.
How does dorsomorphin work as an AMPK inhibitor?
Dorsomorphin is highly selective against AMPK over other structure related kinases such as protein kinase A, protein kinase C and Janus kinase 3. As an AMPK inhibitor, dorsomorphin is found to reverse the anti-proliferation induced by AMPK signaling in glucose-deprived mouse neural stem cells.
What is the role of dorsomorphin in BMP signaling?
Dorsomorphin. It is a potent, selective, reversible, and ATP -competitive inhibitor of AMPK (AMP-activated protein kinase, EC 2.7.11.31) and a selective inhibitor of bone morphogenetic protein (BMP) signaling. It has a role as an EC 2.7.11.31 \\ { [ hydroxymethylglutaryl-CoA reductase ( NADPH )] kinase\\} inhibitor and a bone morphogenetic protein…
How is dorsomorphin used in the treatment of cancer?
Dorsomorphin is used in promoting specific cell differentiation and inducing cancer cell line autophagy. For animal testing, the water-soluble S7306 Dorsomorphin (Compound C) 2HCl is recommended.