How do you make a HBSS buffer?
- Prepare 800 mL of distilled water in a suitable container.
- Add 8 g of NaCl to the solution.
- Add 400 mg of KCl to the solution.
- Add 140 mg of CaCl2 to the solution.
- Add 100 mg of MgSO4-7H2O to the solution.
- Add 100 mg of MgCl2-6H2O to the solution.
- Add 60 mg of Na2HPO4-2H2O to the solution.
What is in Hanks balanced salt solution?
Hanks’ Balanced Salt Solution (HBSS) is composed of inorganic salts and supplemented with glucose. The solution may be used to wash cells and tissue and to maintain cells in a viable state. The solution is buffered with phosphate and maintains a physiological pH and osmotic pressure.
Can cells survive in HBSS?
Cell viability is not compromised at all while cells are in HBSS since it’s a balanced solution with glucose. Cells are happy enough to survive before they are implanted into the mouse.
What is HBSS used for?
Hanks’ Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation.
How do you dilute 10X HBSS?
HBSS, 10X also provides a soure of water, essential inorganic ions, and carbohydrates for the cell or tissue culture. HBSS, 10X is also known as Hanks′ Balanced Salt Solution. Usage : Dilute to 1X concentration using sterile water.
What’s the difference between DPBS and PBS?
PBS is a commonly used buffer with a simple formulation, while DPBS also includes potassium chloride and is available in a larger variety of formulations, including with or without calcium and magnesium and with or without glucose and pyruvate.
What is HBSS made of?
First formulated by the microbiologist John Hanks, Hanks’ Balanced Salt Solution (HBSS) is composed mainly of inorganic salts, glucose or pyruvate as energy sources, phenol red as a pH indicator, and sodium bicarbonate as a pH stabilizer.
What does HBSS do to cells?
HBSS, Modified (Without Ca++ and Mg++) It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as an energy source.