How do you analyze cell cycle data in FlowJo?
FlowJo provides a simple interface to performing fairly sophisticated DNA/Cell Cycle analysis. To launch the univariate cell cycle model click on the population of interest in the workspace, then select the Cell Cycle task from the Biology Band. The univariate model will appear by default as shown in Figure 1.
How do you compensate in FlowJo v10?
1) To initiate creating a new compensation matrix in FlowJo, select the compensation group in the workspace and go to the Workspace ribbon. 2) Click the compensation icon in the Cytometry band of the Tools tab. 3) The compensation interface will launch.
What is FlowJo used for?
FlowJo software is used for the analysis of flow cytometry data. You can use FlowJo to analyze all of your flow cytometry data – regardless of the cytometer used to collect your data files.
How do you use tSNE in FlowJo?
Basic Operation:
- Open FlowJo v10.
- Select/highlight a sample or gated population node within the samples pane of the FlowJo workspace.
- Under the Populations menu (Workspace Tab –> Populations Band), select tSNE.
- Select the parameters to be used for the tSNE calculation.
- Adjust settings (optional).
How many cells are in the cell cycle analysis?
You should analyze 500 000 – 1 000 000 cells. If you determine influence of detrimental factors on the cell cycle, the cell count can be reduced …
How does compensation work in flow cytometry?
The term “compensation,” as it applies to flow cytometric analysis, refers to the process of correcting for fluorescence spillover, i.e., removing the signal of any given fluorochrome from all detectors except the one devoted to measuring that dye.
How do I manually adjust compensation in FlowJo?
You can edit the compensation matrices directly from FlowJo, using the Platform-> Compensate Sample-> Edit/Save Matrix command (from the Platform -> Compensate Sample menu, you can also Remove Compensation).
What is FlowJo v10?
Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data. The environment is presented as the Workspace, which contains a list of loaded samples (experimental data), statistics, gates, and other analyses, as well as tabular and graphical layouts.
How do I add plugins to FlowJo?
Installing Plugins in FlowJo v10:
- Create a folder named “plugins” on your computer.
- Add your plugin of interest to the plugins folder on your machine, and restart FlowJo:
- Plugin actions can be accessed and initiated from within FlowJo under the Plugins Menu. (
What is a TSNE plot?
t-distributed stochastic neighbor embedding (t-SNE) is a statistical method for visualizing high-dimensional data by giving each datapoint a location in a two or three-dimensional map.