What is NucBlue?
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue® Live ReadyProbes® Reagent we have formulated this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle.
Is DAPI a NucBlue?
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA.
What does NucGreen dead bind to?
NucGreen™ dead-cell nucleic acid stain is a cell-impermeant green fluorescent stain exhibits bright, green fluorescence upon binding to nucleic acids. This dye is provided as 1 mM solution in DMSO.
Are used to stain living cells?
Hoechst stains – two types of fluorescent stains, 33258 and 33342, these are used to stain DNA in living cells. Malachite green – a blue-green counterstain to safranin in Gimenez staining for bacteria. This stain can also be used to stain spores. Methylene blue – stains animal cells to make nuclei more visible.
Is Hoechst toxic?
Dyes that bind to DNA, such as Hoechst 33342, are commonly used to visualize chromatin in live cells by fluorescence microscopy. A caveat is that the probes themselves should not perturb cellular responses and under normal conditions the dyes are generally non-toxic.
Is Hoechst a fluorescent?
Thermo Scientific Pierce Hoechst 33342 Fluorescent Stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. …
What is Hoechst used for?
Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2′-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content.
What does DRAQ5 stain for?
DRAQ5™ is a far-red emitting, anthraquinone compound that stains nuclei in live cells. It is permeant to live cells and thus can be used for cell cycle analysis and/or staining of nucleated cells.
How is nucblue used in fixed cell imaging?
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue® Fixed Cell Stain is ideal for use with antibody-based applications. For Research Use Only. Not for use in diagnostic procedures. Immunocytochemistry with HUVEC cells while utilizing the sensitive of Qdot® and convenience of ReadyProbes™.
How is nucblue excited by the UV light?
• NucBlue® Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set.
Which is a negative control for nucblue dye?
Mouse anti-human CD45 antibody shows the leukocytes (red) and NucBlue dye label the nuclei (blue). Explants were either exposed to medium (without LSP), SIVmac251, or 25% LSP. An explant incubated with an isotype control antibody and exposed to LSP is shown as a negative control.
What kind of filter is used for nucblue live cell stain?
• NucBlue® Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine® Alexa Fluor® 350 Dye filter set. For Research Use Only.