What is TaqMan master mix?
TaqMan™ Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan™ Assay-based real-time PCR. Just add your sample and TaqMan Assay, and start your reactions.
What is TaqMan gene expression assays?
TaqMan® Gene Expression Assays are a comprehensive collection of over 600,000 pre-designed probe and primer sets that let researchers quickly and easily perform quantitative gene expression studies on human, mouse, rat, Arabidopsis, or Drosophila genes.
What is Universal PCR Master Mix?
The TaqMan® Universal PCR Master Mix combines components of the TaqMan® PCR Core Reagents Kit with additional key components in one easy-to-use premix. The Taqman® Master Mix also contains an enzyme, AmpErase® Uracil N-glycosylase (UNG), which protects against carry-over contamination.
What is the purpose of master mix in PCR?
The master mix enables researchers to set up controls and test different concentrations of their target DNA or RNA templates without having to individually add precise amounts of enzymes, buffers, cofactor (usually MgCl2), water and dNTP to each reaction tube or plate well.
Can you vortex TaqMan probes?
Multiple assays can be run on one reaction plate. Thaw TaqMan® Assays on ice, then vortex and briefly centrifuge to resuspend.
What is purpose of TaqMan gene expression Master Mix?
Purpose TaqMan ® Gene Expression Master Mix is a convenient mix of components (except primers, probes, template, and water) necessary to perform real-time quantitative polymerase chain reaction (PCR). Use TaqMan Gene Expression Master Mix with the DNA target of your choice, including cDNA, genomic DNA, or plasmid DNA.
How are gene expression assays stored and diluted?
Storage and dilution. • Store TaqMan Gene Expression Assay and TaqMan Non-coding RNA Assay products at –15 to –25 °C and keep them protected from light. • To minimize freeze-thaw cycles, consider diluting 60✕ assays to 20✕ working stocks and dividing the solutions into smaller aliquots.
Which is the best gene expression assay for PCR?
TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed.