Can you reuse ultracentrifuge tubes?
Sealed tubes running in vertical or near-vertical rotors at higher g forces will always undergo some deformation during the run. Do not re-use those.
How do you clean polycarbonate tubes?
Cleaning
- All tubes should be hand-washed with a mild detergent such as Solution 555™ diluted 5-to-1 or 10-to-1 with water.
- Polycarbonate tubes and bottles should not be exposed to detergents with a pH higher than 8.
Can you autoclave polypropylene centrifuge tubes?
Polypropylene tubes can be autoclaved and will resist temperatures up to 121 °C; they will also resist acids, solvents and alkalies at room temperature.
How much does an ultracentrifuge cost?
Ultracentrifuge – $10,000 to $50,000.
Are Falcon tubes sterile?
Falcon® polystyrene Plug Seal screw caps for 50mL centrifuge tubes are sterile. The caps are sold 50 per pack and 1,000 per case.
Can you autoclave Falcon tubes?
You can try with Falcon BD Tubes (now Corning), sterile and autoclavable, but without ventilation caps. Some companies have plastic test tubes and culture vessels, you can try those.
Can you autoclave plastic conical tubes?
DO NOT autoclave capped or sealed tubes or bottles. Do not autoclave tube or bottle accessories made of Noryl (a registered trademark of GE Plastics). Flammable; do not use in or near operating centrifuges.
How many times can you autoclave polypropylene?
Polypropylene (PP) and polypropylene copolymer (PPCO) products can be autoclaved many times. We have PP containers in our lab that have been autoclaved over 100 times and, although they have yellowed somewhat, they are still in service.
What’s the difference between boiling tube and test tube?
Test tubes are convenient containers for heating small amounts of liquids or solids with a Bunsen burner or alcohol burner. A boiling tube is a large test tube intended specifically for boiling liquids.
What is the difference between high speed centrifuge and ultracentrifuge?
is that ultracentrifuge is a high-speed centrifuge, especially one free from convection that is used to separate colloidal particles while centrifuge is a device in which a mixture of denser and lighter materials (normally dispersed in a liquid) is separated by being spun about a central axis at high speed.
Why is polyallomer used in ultracentrifuge tubes?
Polyallomer offers the best overall chemical resistance of plastic ultracentrifuge tubes. It has a useful but limited solvent resistance to alkaline gradients and can be used in a 2-12 pH range. It is transluscent but achieves some contact clarity with liquids.
Can a polyallomer be reused in a centrifuge?
The useful centrifuge temperature range is 0-30 deg C, but short duration runs under certain circumstances may allow the use of tubes up tp 40 deg. C. Polyallomer tubes may be reused (1). Seton polyallomer tubes can be sterilized by autoclaving at 121 deg. C with no detrimental effects on centrifuge tube performance.
Is there a way to sterilize Seton polyallomer tubes?
Seton polyallomer tubes can be sterilized by autoclaving at 121 deg. C with no detrimental effects on centrifuge tube performance. Sterilization can also be achieved by treatment with ethylene oxide gas or by cold methods (2). Seton polyallomer tubes are easily punctured and sliced.
Can a Seton polyallomer rotor be reused?
Seton polyallomer tubes are easily punctured and sliced. Puncturing through the tube bottom is relatively easily. (1) Tubes can be reused if significant deformation or stress cracking has not occurred. Capped tubes used in fixed-angle rotors may deform below the cap and in that case should not be used.