What is the independent variable in the brine shrimp experiment?
In this experiment, what is the dependent variable? (It is the number of eggs to hatch.) What is the independent variable? (Salinity.)
What influences brine shrimp hatching?
The rate of hatching also depends on salinity and pH: the less salt in the water, the easier it is for brine shrimp to absorb enough water to burst through their protective shell. But, specific gravity should be at least 1.011 (15ppt) and can be as high as 1.030 (40ppt).
How does the salinity of water affect the hatching of brine shrimp hypothesis?
The petri dish containing a 2% salinity solution exhibits the highest hatching viability. The petri dish containing a 0% salinity solution has a 0% hatching viability. These results indicate that brine shrimp will not hatch in environments containing no salt and thrive in environments of higher salt concentration.
How could a laboratory researcher determine if there are differences among the brine shrimp that hatched in the solutions of 0% 1% and 2% sodium chloride?
How could a lab researcher determine if there are difference among the brine shrimp in the solutions? They have access to DNA analysis and can look at their phenotypes and such to determine physical characteristics of the brine shrimp.
What is the importance of shrimp brine analysis?
Brine shrimp are aquatic crustaceans belonging to a genus of Artemia. This organism is widely used for testing the toxicity of chemicals. In this study, brine shrimp were evaluated as an infection model organism to study bacterial virulence.
What environmental conditions are needed to hatch brine shrimp eggs?
Hatching Environment
- Salinity: Approximately 11/2 tablespoons of salt per quart (or liter) of water.
- pH: Proper pH is important in hatching brine shrimp.
- Temperature: Optimum water temperature for a 24-hour complete hatch is 80-82°F.
- Light:
- Aeration:
What pH do brine shrimp live in?
A pH of around 8 is best; a pH less than 5 and greater than 10 will likely kill the brine shrimp. The pH can be increased with baking soda, and lowered with muriatic (hydrochloric) acid.
Which salt concentration environment yielded the greatest number of hatched brine shrimp eggs?
Results. Brine shrimp do best at a salinity of 1 2/3 tablespoons of salt per quart of water.
What salinity is best for brine shrimp?
35-40 ppt
The preferred salinity range for culturing brine shrimp is 35-40 ppt (specific gravity 1.024-1.028). Unlike in the preparation of hatching solutions, where household brands of baking salt, kosher salt, and solar salt are adequate, culture water should be pre-mixed using an aquarium-grade marine salt.
What is the principle of brine shrimp assay?
The brine shrimp lethality assay (BSLA) is a simple and inexpensive bioassay used for testing the efficacy of phytochemical present in the plant extracts. The present study determined that the extent of lethality was directly proportional to the concentration of the extract.
What is the principle behind brine shrimp assay as a toxicity test?
Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of bioactive chemicals. It is based on the killing ability of test compounds on a simple zoological organism-brine shrimp (Artemia salina). [1] This assay was first proposed by Michael et al., and further developed by several groups.
How does the brine shrimp hatching experiment work?
Brine Shrimp Hatching Experiment Summary In the first week of this 2-week lab students design an experiment to test how environmental factors might influence the hatching of brine shrimp. The shrimp are raised under different control and treatment conditions and in the second week the students determine the effect of the different treatments
Why are brine shrimp dying in the water?
Many animals like birds eat small animals that live in the water. If the water is polluted, the shrimp may not be as successful, or they may die. You can try other experiments using tiny amounts of cooking oil or dish soap to see how brine shrimp hatching rates change when there is pollution in the water.
How are brine shrimp related to other crustaceans?
Make your own seawater and discover how salt helps or hinders hatching. Brine shrimp are tiny crustaceans that live in salt water. This means that they’re related to crabs, lobsters, and other shrimp. However, brine shrimp are much smaller than other shrimp, and they make intriguing pets.
Where do brine shrimp live in the world?
Name: Date Period: Brine Shrimp (Artemia franciscana) are small shrimp that live in extremely salty water such as The Great Salt Lake in Utah. Because they live in salty water, these shrimp are not considered aquatic species (remember that aquatic = freshwater). They also do not live anywhere in Hawaii.