How to prepare of polyplexes?
Briefly, polyplexes were prepared by adding a complexation buffer solution (20 mM HEPES, 5 wt% glucose, pH 7.4) of polymer (844 μg mL−1 for DNA, 1125 μg mL−1 for RNA) to a complexation buffer solution of nucleic acid (75 μg mL−1) followed immediately by 20 seconds of vortex mixing.
What is the meaning of Lipoplexes?
Lipoplexes are defined as the complexes among plasmid DNA and cationic lipids. The formation of the complex is due to the electrostatic interaction between the polar headgroups of cationic lipids and the phosphate groups of DNAs.
What are Lipoplexes and Polyplexes?
Lipoplexes and polyplexes are colloidal suspensions or complexes of DNA condensed with cationic lipids and polymers, respectively, which have shown significant instability in the extracellular compartment of the body.
How Does gene therapy work step by step?
With gene therapy, doctors deliver a healthy copy of a gene to cells inside the body. This healthy gene may replace a damaged (mutated) gene, inactivate a mutated gene or introduce an entirely new gene. Carriers, called vectors, transport these healthy genes into cells.
Why are viruses used in gene therapy?
Certain viruses are often used as vectors because they can deliver the new gene by infecting the cell. The viruses are modified so they can’t cause disease when used in people. Some types of virus, such as retroviruses, integrate their genetic material (including the new gene) into a chromosome in the human cell.
What are polyplex and Lipoplex?
What are the functions of a Polyplex system?
Polyplexes are polymeric systems containing condensed and/or complexed gene or siRNA through electrostatic interactions between cationic groups of the polymer and the negatively charged nucleic acids. They protect nucleic acids from enzymatic degradation and enable cargo release to tumor sites (Prabhu et al., 2015 ).
How are cationic polymers used to create polyplexes?
Cationic polymers are commonly used to complex with nucleic acids having negatively charges phosphate backbone, forming polyplexes, the stability of which depends on the ratio of positive to negative charges (N/P). For example, linear PEI/plasmid complexes were less stable than branched PEI/plasmid complexes (Madani et al., 2011).
What is the dividend rate of Polyplex Corporation?
Polyplex Corporation Limited has submitted to the exchange, the financial results for the period ended June 30, 2021. The Board of Directors of the Company have declared payment of Interim Dividend for the Financial Year 2021-22 @ Rs.33/-per share (of the face value of Rs.10/-each), subject to TDS / Withholding Tax.
How are polyplexes formed in aqueous conditions?
Polyplexes are usually formed in aqueous conditions by pipetting a solution of the studied polymer or dendrimer into a solution of the polynucleotide of study and allowing the solution to ‘incubate’ or sit for a certain length of time to allow electrostatic binding, complexation, and formation of nanoparticles.