What is multiplexing in biology?
In the biological sciences, a multiplex assay is a type of immunoassay that uses magnetic beads to simultaneously measure multiple analytes in a single experiment. In a multiplex assay, microspheres of designated colors are coated with antibodies of defined binding specificities.
What does multiplexing mean in DNA sequencing?
Sample multiplexing, also known as multiplex sequencing, allows large numbers of libraries to be pooled and sequenced simultaneously during a single run on Illumina instruments. Sample multiplexing is useful when targeting specific genomic regions or working with smaller genomes.
What is multiplexing RNA seq?
The need to reduce per sample cost of RNA-seq profiling for scalable data generation has led to the emergence of highly multiplexed RNA-seq. These technologies utilize barcoding of cDNA sequences in order to combine multiple samples into a single sequencing lane to be separated during data processing.
What is cell multiplexing?
Cell Multiplexing refers to the labeling of a cell or nuclei sample with a molecular tag and subsequently mixing this sample with other labeled samples. This set of multiplexed samples can be processed together in a single GEM well.
What are some ways in which DNA sequencing is used?
Sequencing is used in molecular biology to study genomes and the proteins they encode. Information obtained using sequencing allows researchers to identify changes in genes, associations with diseases and phenotypes, and identify potential drug targets.
What is multiplexing in RT PCR?
Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.
What is sequencing library?
A sequencing library is, by definition, a pool of DNA fragments with adapters attached. Adapters are designed to interact with a specific sequencing platform, either the surface of the flow-cell (Illumina) or beads (Ion Torrent).
What is single cell hashing?
Cell Hashing is a method that enables sample multiplexing and super-loading on single cell RNA-sequencing platforms, developed in the Technology Innovation lab at the New York Genome Center in collaboration with the Satija lab.
What is feature barcoding?
“Feature Barcoding” refers to using antibodies linked to specific oligos. The antibodies can then bind to cell surface markers of interest and the oligos they’re conjugated with turned into normal scRNA-seq reads.
What does it mean to Multiplex Control Files?
Multiplexing is the process of mintaining a copy of same control files on different disk drivers (and idealy on different controllers). To multiplex your control files, we copy the control file to multiple locations and change the CONTROL_FILES parameter in the text based initialization file init.ora to include all control files names. 1.
Is there a way to multiplex Oracle control files?
Answer: There are two ways to multiplex the oracle control files, init.ora and spfile. Multiplexing is the process of mintaining a copy of same control files on different disk drivers (and idealy on different controllers).
What are the advantages of multiplex qPCR experiments?
The many advantages of multiplex qPCR experiments arise from the fact that the assays for the test and the control gene use the same reagents in the same reaction. These reactions are, however, in competition for the same limited pool of reagents.
What is the general procedure for multiplex reactions?
The general procedure is as follows: Run your singleplex reactions and confirm amplification. After establishing conditions for performing singleplex reactions, set up conditions for multiplex reactions. Ensure amplification occurs and analyze data. Determine whether the singleplex and multiplex reactions give the same Ct values.