What is the pI for myoglobin?
The pI of the myoglobin is estimated to be in the range 6.5–7.0.
What is the isoelectric pH of cytochrome C?
1.7 pH-units
The isoelectric point of cytochrome c in literature has inaccuracy of 1.7 pH-units.
What is the pI of hemoglobin?
A precipitate brought about by the mutual aggregation of hemoglo- bin and stroma material is formed, since at pH 5.5 hemoglobin is on the acid side of its isoelectric point (6.8) and stroma material is on the alkaline side of its isoelectric point (about 3.0) (cj.
What is the extinction coefficient of myoglobin?
The concentration of myoglobin ranged from approximately 0.25 to 0.5 mM. The extinction coefficient for myoglobin from horse at 408 nm is 188 mM−1 cm−1.
What is the main function of myoglobin?
myoglobin, a protein found in the muscle cells of animals. It functions as an oxygen-storage unit, providing oxygen to the working muscles. Diving mammals such as seals and whales are able to remain submerged for long periods because they have greater amounts of myoglobin in their muscles than other animals do.
What is the pI of lysozyme?
= 11.1
Because lysozyme has a very high pI (pI = 11.1), back-extraction of lysozyme in the AOT system requires that the aqueous phase have a at very high pH and high salt concentration.
What is the molecular weight of myoglobin?
approximately 17 kDa
Myoglobin is a small cytoplasmic heme-containing protein with a molecular weight of approximately 17 kDa, which is found in both skeletal and cardiac muscle.
What is the process of hemoglobin?
Hemoglobin in blood carries oxygen from the lungs or gills to the rest of the body (i.e. the tissues). There it releases the oxygen to permit aerobic respiration to provide energy to power the functions of the organism in the process called metabolism.
Does myoglobin dissolve in water?
This protein is soluble in water (10 mg/ml), yielding a clear, red brown solution.
What is normal myoglobin level?
Normal Results The normal range is 25 to 72 ng/mL (1.28 to 3.67 nmol/L). Note: Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or may test different samples. Talk to your provider about the meaning of your specific test results.
How is hemoglobin different from myoglobin?
Hemoglobin is a heterotetrameric oxygen transport protein found in red blood cells (erythrocytes), whereas myoglobin is a monomeric protein found mainly in muscle tissue where it serves as an intracellular storage site for oxygen.
What is the pI of the myoglobin protein?
The pI of the myoglobin is estimated to be in the range 6.5–7.0. b – d, The same as in a but for BSA (b), fibrinogen (c) and RNase A (d). The pI of BSA, fibrinogen and RNase A is estimated to be in the range 4.5–5.0, 5.5–6.0 and 9.5–10.0, respectively.
What do you need to know about myoglobin blood test?
Myoglobin, Serum Test. also known as: Urine Myoglobin , Serum Myoglobin. Myoglobin is a protein that is present in your heart and skeletal muscles. It captures the oxygen required for your muscles to function. Occurrence of a heart attack or severe muscle damages leads to the release of myoglobin into the blood stream.
How long does it take for myoglobin to go up?
Measurement of myoglobin provides an early index of damage to the myocardium, such as occurs in myocardial infarction (MI) or reinfarction. Increased levels, which indicate cardiac muscle injury or death, occur in about 3 hours. Although this test is more sensitive than creatine phosphokinase (CPK) isoenzymes, it is not as specific.
How much does myoglobin test cost in India?
Myoglobin, Serum Test. Average price range of the test is between Rs.800 to Rs.4000 depending on the factors of city, quality and availablity.