Is CTAB soluble in water?
According to the MSDS: CTAB’s solubility in water is 36.4 g/l at 20 °C (68 °F) – completely soluble. It shouldn’t require anything special.
What is the cmc of CTAB?
The CMC of CTAB is 0.0009 mol/L in water, while it is 0.24 mol/L in ethanol. Furthermore, the dissipative particle dynamics (DPD) was adopted to simulate the aggregation of CTAB in water and ethanol/water mixtures, and the energy difference was calculated for the surfactant tail groups after mixing with the solvent.
Is CTAB a surfactant?
Cetrimonium bromide ([(C16H33)N(CH3)3]Br; cetyltrimethylammonium bromide; hexadecyltrimethylammonium bromide; CTAB) is a quaternary ammonium surfactant. It is one of the components of the topical antiseptic cetrimide.
Is CTAB light sensitive?
The material can produce chemical burns to the eye following direct contact. Vapors or mists may be extremely irritating. If applied to the eyes, this material causes severe eye damage. Direct eye contact with acid corrosives may produce pain, tears, sensitivity to light and burns.
How do you make a CTAB solution?
Make a 10% CTAB working solution (50 mL): Combine 50 mL of 0.7 M NaCl and 2.5 g of CTAB into a 50 mL polypropylene tube (Fisher Scientific cat no. 06-443-18). Rotate slowly at 60˚C for several hours to dissolve powder completely. Store at Room Temperature for up to 6 months.
What is CTAB solution?
CTAB (also called hexadecyltrimethylammonium bromide) is a cationic detergent that facilitates the separation of polysaccharides during purification while additives, such as polyvinylpyrrolidone, aid in inactivating polyphenols. CTAB based extraction buffers are widely used when purifying DNA from plant tissues.
How do you dissolve CTAB in water?
While stirring, add 10 g CTAB. To dissolve, heat the solution at 65ºC. Adjust the volume to 100 mL with MilliQ water. Store at RT for no longer than 6 months.
What is the molecular weight of CTAB?
364.45 g/mol
Cetrimonium bromide/Molar mass
Is CTAB hydrophobic?
The packing of the CTAB molecules likely forms strongly hydrophobic environments produced by the long C16H37 chains inside mesoporous cavity. The EE molecule is fairly hydrophobic (water solubility of 20 mg L−1, 20 °C) and should interact very well with these hydrophobic environment.
What type of surfactant is CTAB?
What should be the pH of CTAB buffer?
2 % CTAB. 100 mM Tris (pH 8.0) 20 mM EDTA.
How do you dissolve CTAB?
Dissolve 4.1 gram NaCl (Merck, p.a.) in 80 mL distilled water. While stirring, add 10 gram N-cetyl-N,N,N,-trimethyl ammonium bromide (CTAB) (Merck, p.a.). To dissolve heat the solution at 65 °C. Adjust the volume to 100 mL with distilled water.
What is the solubility of CTAB in water?
Cetyltrimethylammonium bromide (CTAB) (Sigma–Aldrich) (purity >98%) was purified by the method described in the literature [14]. The solubility of CTAB in water at 293 K is equal to 3 g/dm 3 (≈0.008 M).
Why does CTAB CMC increase with ethanol concentration?
[4] investigated the CTAB–ethanol system in the whole range of ethanol concentration and they stated that the CMC of CTAB increases with the increasing content of alcohol in the solution because of the stronger interactions of CTAB hydrophobic tail with ethanol than with water which makes micellization more difficult.
When to use cetyltrimethylammonium bromide ( CTAB )?
Cetyltrimethylammonium bromide (CTAB) is a cationic detergent used in the isolation of high molecular weight DNA. CTAB is used to precipitate DNA. read more → Also soluble in chloroform. Slightly soluble in acetone.
How are mixed micelles of CTAB and methanol formed?
The mixed micelles of CTAB and methanol are probably formed in the whole range of alcohol concentration but those of CTAB and ethanol or 1-propanol only in their concentration range in which they are present in the monomeric form in the solution.