Is calcein AM toxic to cells?
Calcein, AM has been used for studies of enzymatic activity, cell membrane integrity, and long-term cell tracking due to its low cellular toxicity. It is important to note that for certain cell lines and experimental conditions, relatively high concentrations of Calcein, AM can be toxic to cells.
What is calcein AM used for?
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases.
How do you store calcein am?
Store Calcein AM Reagent at -20°C and the rest of the kit at +4°C on receipt. Δ Note: Once reconstituted with DMSO, use Calcein AM immediately, or store at ≤-20°C for up to 6 months, protected from light and thawed no more than twice during that time.
How do you take calcein?
Add 100 µl of working stock solution of Calcein AM dye and incubate the cells for 30 minutes or 1hr in incubator (5%CO2, 37°C). NOTE: For most cell types, 30 minutes incubation is adequate.
Does calcein AM stain dead cells?
Calcein AM is an excellent tool for distinguishing live and dead cells, as well as for cell viability measurement. Since dead cells lack esterase activity, only live cells are stained and detected.
Is calcein am light sensitive?
Calcein AM Dye is supplied at ambient temperature. Upon arrival store at -20°C. The product when stored as directed is stable for 1 year. light as Calcien AM dye is light sensitive.
How do you dilute calcein?
Each 50 µg of Calcein-AM may be reconstituted with 50 µL of anhydrous DMSO to yield a stock concentration of 1 mM.
What do you need to know about Invitrogen?
Invitrogen. Invitrogen™ cell and molecular biology products range from do-it-yourself reagents and optimized kits to connected instruments, all working together holistically to enable scientists to do what they do best—only better. Please log in to view your contract price information for any cross-referenced items.
Which is Thermo Scientific Invitrogen NanoDrop and Qubit 4?
Thermo Scientific™ Invitrogen™ Nanodrop™ One Spectrophotometer with WiFi and Qubit™ 4 Fluorometer As a bundled offering, the NanoDrop™ One Spectrophotometer and Qubit™ 4 Fluorometer allow you to obtain the most complete information about the concentration and quality of your DNA, RNA, or protein samples. 29
How is celltrace cell proliferation kit used in vivo?
CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. • Superior performance—bright, single-peak staining enables visualization of multiple generations. • Long-term signal stability—well-retained in cells for several days post stain.
When was Life Technologies acquired by Thermo Fisher Scientific?
Life Technologies was acquired by Thermo Fisher Scientific in January, 2014 Our innovative life science, applied science, and clinical products are now found under the Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, and Molecular Probes™ brands.