What is a bpg column?

What is a bpg column?

BPG columns are glass chromatography columns designed to meet the needs of process development and biopharmaceutical manufacture; hygienic design and operation; easy, efficient packing and running with the single-screw adapter.

What is slurry in column chromatography?

For the wet method, a slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. During the entire chromatography process the eluent is collected in a series of fractions. Fractions can be collected automatically by means of fraction collectors.

What is packing height?

The height of a transfer unit (HTU) is a measure of the separation effectiveness of the particular packings for a particular separation process. Determination of the packed height can be based on either the gas-phase or the liquid-phase.

Who is the father of chromatography?

Mikhail Tsvet
Chromatography/Inventors
1.1. 1 Who is the Father? Chromatography was invented by the Russian botanist Mikhail Semenovich Tswett during his research on the physicochemical structure of plant chlorophylls.

How do you improve separation in column chromatography?

Depending on the situation, separations can sometimes be improved by increasing the column plate number, by using smaller particles or by increasing column length. The disadvantages of these approaches are higher operating pressures and increased separation times for longer columns.

What is wet and dry packing of column?

In the “dry packing” method, dry silica or alumina is added directly to a column, and solvent is allowed to trickle through in portions, then with pressure. In the “wet packing” method, the column is filled with solvent first, then dry silica or alumina is lightly shaken in, then packed with pressure.

What is column performance?

Column performance — the performance of a chromatography column may be determined by measuring the number of theoretical plates (N). These theoretical plates are hypothetical stages where substances come into equilibrium. The greater the number of theoretical plates for a column, the more efficient the separation.

Who invented TLC?

Thin layer chromatography (TLC), as shown on the right, was developed in 1938 by Izmailov and Shraiber [3] , based on Mikhail Tswett’s earlier description of column chromatography.

Who first used chromatography?

botanist Mikhail Tsvet
The first true chromatography is usually attributed to the Russian-Italian botanist Mikhail Tsvet. Tsvet applied his observations with filter paper extraction to the new methods of column fractionation that had been developed in the 1890s for separating the components of petroleum.

What kind of column is BPG 140 / 500?

BPG 140/500 Column Glass chromatography columns for process development or biopharmaceutical manufacture, designed for hygienic operation and easy, efficient packing.

What kind of clamp do you need for a BPG column?

For storage purposes, the 25 mm blind flange with a clamp and gasket can be used to seal off the column. A clamp and gasket, 6 or 10 mm internal diameter, are required to connect the 25 mm sanitary flanged inlet/outlet to either valves or tubing of the same type. Preflanged tubing in 6 and 10 mm internal diameter is available.

Which is the BPG 100 stand with wheels?

Stand kits must be ordered separately. BPG 100 stand kit has adjustable feet; wheels with brakes are available: product code 18-1031-10. BPG 140 and 200 stands have wheels with brakes as standard: product code 18-1031-20. BPG 300 and 450 are supplied with stainless steel stand with wheels and foot-operated brakes.

What should be included in a BPG air trap?

Air traps: BPG Air Trap Complete includes the air trap, mounting bracket, steel valves, clamps, and gaskets. For air traps for BPG 100, 140, and 200, tubing is included. Top valve: Manually operated valve recommended at the top of the air trap as an air outlet control.