What is the specificity of the antibody LC3B?
Specificity / Sensitivity. LC3B detects endogenous levels of total LC3B protein. Cross-reactivity may exist with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B.
How is LC3 converted to LC3-II during autophagy?
During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).
How long to incubate LC3 antibody for WB?
WB result of LC3 antibody (14600-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-LC3 transfected HeLa cells. WB result of LC3 antibody (14600-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-LC3 transfected HeLa cells.
Which is the type II form of LC3B?
Cross-reactivity may exist with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B.
Where is LC3 found in the human body?
Microtubule-associated protein 1A/1B-light chain 3 (MAP1LC3), commonly shortened to LC3, is a soluble protein with a molecular weight of approximately 17kDa. LC3 is distributed throughout mammalian tissues and cultured cells 1 and is a key component of autophagy, the recycling system of the eukaryotic cell.
Which is an autophagosome marker for LC3 antibody?
LC3 Protein and Autophagy. The mammalian homolog of Atg8 is called microtubule-associated proteins 1A/1B light chains 3A/LC3A and 3B/LC3B (MAP1LC3A/B), abbreviated to LC3. These ubiquitin-like proteins can be used as reliable autophagosome markers for monitoring autophagy.
Is there an anti LC3 antibody for rabbits?
Anti-LC3-I/II, Cat. No. ABC929, is a rabbit polyclonal antibody that detects LC3B-I/II and is tested for use in Immunocytochemistry and Western Blotting. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LC3-I/II in HeLa cells treated with 100 mM Chloroquine.