What is the gene that codes for kanamycin resistance?
All hybrid plasmids contain the promoterless part of kanamycin resistance gene (which codes for aminoglycoside 3′-phosphotransferase II) from transposon Tn5. The Km gene expression is driven by a promoters situated on pLD720.
What is the mechanism of action for kanamycin?
Kanamycin is an aminoglycoside antibiotic used to treat different types of bacterial infections. Kanamycin works by binding to the bacterial 30S ribosomal subunit, causing misreading of mRNA and leaving the bacterium unable to synthesize proteins vital to its growth.
What does kanamycin do to E coli?
The aminoglycoside antibiotic kanamycin damages DNA bases in Escherichia coli: caffeine potentiates the DNA-damaging effects of kanamycin while suppressing cell killing by ciprofloxacin in Escherichia coli and Bacillus anthracis. Antimicrob Agents Chemother.
How does kanamycin inhibit cell growth?
Kanamycin is an aminoglycoside antibiotic. Aminoglycosides work by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth.
What part of the cell does kanamycin target?
Kanamycin A belongs to the family of aminoglycoside antibiotics that target cellular RNA to inhibit bacterial and viral replication. Previous studies have shown that aminoglycosides bind to mammalian but disrupt bacterial membranes.
What cellular component does kanamycin target?
Kanamycin A belongs to the family of aminoglycoside antibiotics that target cellular RNA to inhibit bacterial and viral replication.
How does kanamycin target the cell?
Kanamycin A belongs to the family of aminoglycoside antibiotics that target cellular RNA to inhibit bacterial and viral replication. Computational studies revealed strong hydrogen bonding interactions between the hydroxyl and amino groups of the aminoglycoside with the ester carbonyl and phosphate groups of the lipids.
How are kanamycin and hygromycin resistance related?
The nptII gene confers transgenic plants resistance to the antibiotic, kanamycin, kan, and the hptII gene provides transgenic plants resistance to the antibiotic, hygromycin, hyg [3,5]. These antibiotics normally inhibit protein synthesis in plants by binding to ribosomes [5].
How is kanamycin used in molecular biology research?
Use in research. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]).
What makes MTB resistant to amikacin and kanamycin?
An A1400G mutation of the rrs gene was identified in Mycobacterium tuberculosis (MTB) strain ATCC 35827 and in 13 MTB clinical isolates resistant to amikacin-kanamycin (MICs, >128 microg/ml). High-level cross-resistance may result from such a mutation since MTB has a single copy of the rrs gene.
What is the resistance mechanism of kanamyceticus isp5500?
In these cases, the inactivating enzymes were found to have a major role in the resistance mechanism. However, the resistance of S. kanamyceticus ISP5500 to streptomycin seems to be due to resistance at the ribosomal level.