Does all MRSA have mecA gene?
A major determinant is the mecA gene, found in all MRSA strains, which encodes a novel penicillin-binding protein PBP 2a. The 78 kDa PBP 2a protein shows a very low affinity for penicillin, but is still capable of assembling peptidoglycan.
Where is the mecA gene located?
And the mecA gene is the coding gene of PBP2′, and located in the SmaI fragment G of the chromosome map by Pattle P.A.,. A part of the structure of mecA is similar to that of the penicillinase gene.
What is blaZ gene?
blaZ is an 846-bp gene controlled by two regulatory genes (antirepressor blaR1 and repressor blaI) [3]. After exposure to β-lactams, blaR1 (transmembrane sensor–transducer) undergoes autocatalytic cleavage, promoting the cleavage of blaI and leading to the transcription of blaZ [1,4].
Does the presence of the mecA gene confirm MRSA?
MRSA is resistant to all β-lactams because of the presence of mecA, a gene that produces a pencillin binding protein (PBP2a) with low affinity for β-lactam antibiotics.
How is mecA gene transferred?
mecA is acquired and transmitted through a mobile genetic element, that inserts itself into the host genome. That structure is conserved between the mecA gene product and a homologous mecA gene product in Staphylococcus sciuri.
What is mecA used for?
mecA is a gene found in bacterial cells which allows them to be resistant to antibiotics such as methicillin, penicillin and other penicillin-like antibiotics.
What is Blaz?
Blaž is a masculine given name found in Slovenia, Croatia and Bosnia and Herzegovina. It is cognate to Blaise. It may refer to: Blaž Arnič, Slovenian composer. Blaž Bertoncelj, Slovene dancer.
What protein does mecA gene encode for?
Resistance to methicillin is determined by the mecA gene, which encodes the low-affinity penicillin-binding protein PBP 2A (3).
What replaced methicillin?
Its role in therapy has been largely replaced by flucloxacillin and dicloxacillin, but the term methicillin-resistant Staphylococcus aureus (MRSA) continues to be used to describe S. aureus strains resistant to all penicillins.