What is flotation technique in parasitology?
FLOATATION TECHNIQUE. Principle. The principle is to use an emulsifying fluid of a greater specific gravity than that of the parasite eggs, which result in flotation of eggs in the solution. Thereby mere examination of the solution in the top most layer will clearly indicate the presence of egg.
What is a centrifugal flotation?
Centrifugal flotation is the best method of detecting common parasites in companion and exotic animals. Becoming familiar with the technique of centrifugal flotation can help veterinary technicians better detect common parasite eggs found in feces but not always seen in fecal flotation.
What is flotation technique in routine stool examination?
Fecal flotation is a routine veterinary test used to diagnose internal parasites or “worms.” The test detects the eggs of mature parasites that live inside the body and pass their eggs to the outside by shedding them into the host’s stool.
What parasite egg can be isolated using flotation technique?
Stool Specimens – Centrifugal Flotation for Intestinal Parasites. Notes on method: Centrifugal flotation effectively concentrates fertilized eggs of Ascaris species, Trichuris species, hookworm, Hymenolepis species and Enterobius vermicularis.
What is flotation technique is used for?
The simple test tube flotation technique is a qualitative test for the detection of nematode and cestode eggs. This is a useful method to use in preliminary surveys to establish which parasite groups are present.
How do you make sheather’s sugar solution?
Heat the tap water to near boiling. Add the granulated sugar, and stir until the sugar is dissolved. Allow the mixture to cool to room temperature, and then add the formaldehyde. Check the solution’s specific gravity, and adjust it to 1.27 by adding water or sugar.
What is formalin-ether concentration technique?
The formalin-ether concentration technique (FECT) is a widely used sedimentation technique for the diagnosis of intestinal protozoa in preserved stool samples (2). The most commonly used fixatives for stool preservation are either formalin or sodium acetate-acetic acid-formalin (SAF) (3, 24, 40).
What is the purpose of flotation technique?
What are the different flotation concentration techniques?
Concentration techniques like simple slat flotation, Zinc sulphate centrifugal floatation, formol-ether concentration and modified formol-ether concentration.
What are the other specimens examined in parasitology?
Other specimens received for detection of parasites include urine, sputum, liver aspirates, duodenal aspirates, bile, corneal scrapings, contact lens fluid, and tissue. The use of serology for the diagnosis of parasitic infections is increasing.
What is mini Flotac technique?
Mini-FLOTAC is a new diagnostic method based on flotation of the eggs. Two chambers (1 ml each) are placed in the base and surmounted by a reading disc. The Mini-FLOTAC is part of a kit together with the fill-FLOTAC, a plastic device used to homogenize, filter and pour the sample in the flotation chambers[11].