What is a Pulse damper for HPLC what is it used for?
The pulse damper 10 is designed for use in a liquid pumping system, such as a liquid chromatography system in which the outflow from a reciprocating pump is delivered in pulses to a flow line leading to a chromatographic column.
What is the pump used for in HPLC?
The purpose of the pump is to force the liquid through the HPLC system while it maintains a constant flow of mobile phase. This occurs regardless of the back pressure caused by the flow resistance of the HPLC column.
What is the detector in HPLC?
HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase.
What is wet prime in HPLC?
Wet prime pumps (often called ‘self-primers’) require the casing to be filled with water prior to the first start-up only. After that, the liquid will always remain in the pump volute, so the suction hose can run dry without damaging the pump or seals.
Why is methanol used in HPLC?
Methanol is a polar-protic solvent, whereas acetonitrile is a polar-aprotic solvent and possesses a stronger dipole moment. This means that the organic modifier used in the mobile phase can have a powerful effect on chromatographic selectivity.
What is d2 lamp in HPLC?
HERAEUS NOBLELIGHT DEUTERIUM LAMPS Deuterium lamps emit an almost continuous spectrum of light ranging from the main UV wavelengths of 160 – 400 nm to the visible spectral range (800 nm). This makes them the ideal light source for high precision absorption measurements, e.g. in HPLC.
What is gradient flow in HPLC?
1 Introduction. Gradient elution in HPLC refers to the technique of altering the composition of the mobile phase during the course of the chromatographic run. 1, except that changes in solvent strength rather than temperature are used to elute the solutes.
What is gradient chromatography?
Definition: A separation method where the components are distributed between two phases, one of which is stationary, while the other moves in a definite direction (the ‘mobile’ phase).