What chromosome is PV92 located on?
chromosome 16
One example of a non-disruptive Alu element in humans is found in the location called PV92 on chromosome 16. This element is of the youngest subfamily of Alu, called Ya5.
What is PV92 and the Alu insert?
The PV92 genetic system has only two alleles indicating the presence (+) or absence (-) of the Alu transposable element on each of the paired chromosomes. Alu is an example of a so-called “jumping gene” – a transposable DNA sequence that “reproduces” by copying itself and inserting into new chromosome locations.
Is the PV92 locus polymorphic?
Some of the human Alu elements have retroposed so recently that their insertion at a specific location within the human genome remains polymorphic. One of these polymorphic Alu insertions is the PV92 Alu site that is located in chromosome 16, and it has been proved to be human-specific (Batzer et al. 1994 ).
What is PV92 locus?
PV92 alleles The DNA region, or locus, that you’ll copy is called PV92; it’s part of an intron on chromosome 16. The result is that for any copy of human chromosome 16, you should be able to amplify either a short (no Alu) or a long (+Alu) PV92 PCR product.
Why can a person be either homozygous or heterozygous for the PV92 locus?
Why can a person be either homozygous or heterozygous for the PV92 locus? A person can have the insertion on both chromosomes (homozygous dominant), not have the insertion on either (homozygous recessive), or have the insertion on only one chromosome (heterozygous).
What is the PV92 locus?
An allele is a particular version of a nucleotide sequence; a PV92 genotype is the set of alleles found in one individual. Since everybody has two copies of chromosome 16 (one from mom and one from dad), everybody has two copies of the PV92 locus.
Is PV92 polymorphic?
Insertion polymorphism The PV92 lab is based on a polymorphism, or a DNA region that varies from one individual to another. In this case, the polymorphism is due to an insertion, which means that an extra piece of DNA (the ALU transposon) is inserted into the genomes of some people, but not others.
What kind of DNA is ALU PV92?
transposon
Alu is a transposon Because transposons tend to get duplicated, they can occur many times in an organism’s genome, and because they tend to get inserted at new locations, these repeated DNA sequences can be scattered throughout the genome.
What gene is PV92?
PV92 genotypes An allele is a particular version of a nucleotide sequence; a PV92 genotype is the set of alleles found in one individual. Since everybody has two copies of chromosome 16 (one from mom and one from dad), everybody has two copies of the PV92 locus.
What is PV92 PCR?
With the PV92 PCR Informatics Kit, your students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus.
Where is the PV92 locus on chromosome 16?
The PV92 locus. The DNA region, or locus, that you’ll copy is called PV92. The PV92 locus is part of an intron on chromosome 16. The thing that makes PV92 useful for DNA fingerprinting is that it’s variable. Everybody has the PV92 locus, but some people have an extra stretch of repetitive DNA (called an Alu repeat) in it.
Is the PV92 ALU a human specific insertion?
One of these polymorphic Alu insertions is the PV92 Alu site that is located in chromosome 16, and it has been proved to be human-specific ( Batzer et al. 1994 ).
Can a PCR primer target the PV92 locus?
As shown in the diagram above, you’ll use PCR primers that target a conserved region of the PV92 locus. The result is that for any copy of human chromosome 16, you should be able to amplify either a short (no Alu) or a long (+Alu) PV92 PCR product. The sizes of the expected PCR products for the two alleles are:
How many PV92 alleles are there in the world?
Everybody has the PV92 locus, but there are two common alleles: with or without an Alu transposon inserted. There are only two common PV92 versions, or alleles, in the human population – the short one, with no Alu repeat, and the long one, with the Alu repeat.