What is TaqMan SNP genotyping?
The TaqMan® SNP genotyping technology utilizes the 5′ nuclease activity of Taq polymerase to generate a fluorescent signal during PCR. For each SNP, the assay uses two TaqMan® probes that differ in sequence only at the SNP site, with one probe complementary to the wild-type allele and the other to the variant allele.
What is genotyping analysis?
Genotyping is the process of determining differences in the genetic make-up (genotype) of an individual by examining the individual’s DNA sequence using biological assays and comparing it to another individual’s sequence or a reference sequence.
How does a genotyping chip work?
In order to be genotyped, the amplified DNA is “cut” into smaller pieces, which are then applied to our DNA chip (also known as a microarray), a small glass slide with millions of microscopic “beads” on its surface. Each bead is attached to a “probe,” a bit of DNA that matches one of the genetic variants that we test.
How does a TaqMan assay work?
TaqMan genotyping assays are used to amplify and detect specific alleles in genomic DNA (gDNA). The exonuclease activity of AmpliTaq Gold® DNA Polymerase cleaves only probes hybridized to the target. Cleavage separates the reporter dye from the quencher dye, increasing fluorescence by the reporter.
How does the TaqMan SNP genotyping assay work?
The TaqMan® SNP genotyping technology utilizes the 5’ nuclease activity of Taq polymerase to generate a fluorescent signal during PCR. For each SNP, the assay uses two TaqMan® probes that differ in sequence only at the SNP site, with one probe complementary to the wild-type allele and the other to the variant allele.
How does Thermo Fisher SNP genotyping assays work?
Genotyping assays aim to discriminate between sequences, rather than measure the level of a particular DNA sequence. If the scatter plot could show the trajectory taken by each data point over the course of the experiment, it would look like a clam shell. This clam-shelling effect only occurs in SNP genotyping assays of homozygous samples.
How are genotyping assays developed for single nucleotide polymorphism?
Each assay allows genotyping of individuals for a single nucleotide polymorphism (SNP). All assays are developed using our bioinformatics assay design processes that apply heuristic rules that are deduced from both manufacturing and assay performance data.
How are genotyping assays used in Applied Biosystems?
Applied Biosystems™TaqMan®SNP Genotyping Assays use TaqMan®5´‑nuclease chemistry for amplifying and detecting specific polymorphisms in purified genomic DNA samples. Each assay allows genotyping of individuals for a single nucleotide polymorphism (SNP).