Why is termination of transcription an Rho factor independent?
Intrinsic, or rho-independent termination, is a process in prokaryotes to signal the end of transcription and release the newly constructed RNA molecule. A protein bound to RNA polymerase (nusA) binds to the stem-loop structure tightly enough to cause the polymerase to temporarily stall.
What is Rho dependent and rho independent termination?
The key difference between Rho dependent and Rho independent termination is that in Rho dependent termination, Rho factor binds with the transcript and terminates transcription by breaking hydrogen bonds between the template and the transcript, while Rho independent termination terminates the transcription by producing …
Does Rho terminate transcription?
Rho terminates transcription in response to specific DNA signals transcribed into RNA called the rho-dependent terminators. There are two fundamental features of a rho-dependent terminator; a proximal Rho binding site called the ‘rut site’ (Rho utilization) and a distal sequence comprising the termination zone.
Which transcription can be terminated by Rho?
Transcription termination factor Rho is an ATP-dependent RNA helicase/translocase, which can bind to a sequence motif called the Rho utilization (rut) site on the nascent transcript, translocate along the naked RNA and dissociate the elongation complex to terminate transcription (Grylak-Mielnicka et al., 2016; Ray-Soni …
What happens in rho-independent termination?
Rho-independent termination depends on specific sequences in the DNA template strand. As the RNA polymerase approaches the end of the gene being transcribed, it hits a region rich in C and G nucleotides. The RNA transcribed from this region folds back on itself, and the complementary C and G nucleotides bind together.
Why does rho-dependent transcriptional termination in bacteria require the rho protein chegg?
Question: Why does rho-dependent transcriptional termination in bacteria require the rho protein? O The rho protein helps unwind the stem-loop structure after the RNA has been released by RNA polymerase. O RNA Polymerase stalls on the termination stem-loop and rho is needed to displace the RNA polymerase.
What do you mean by rho dependent termination of transcription?
Rho dependent termination is one of two types of termination in prokaryotic transcription, the other being intrinsic (or Rho-independent). After binding to the newly formed RNA chain, ρ factor moves along the molecule in a 5′-3′ direction and encourages dissociation from the DNA template and RNA polymerase.
Is rho dependent termination only in prokaryotes?
Rho factor is an essential transcription protein in prokaryotes. In Escherichia coli, it is a ~274.6 kD hexamer of identical subunits. Each subunit has an RNA-binding domain and an ATP-hydrolysis domain….Rho factor.
| Transcription termination factor Rho | |
|---|---|
| UniProt | P0AG30 |
| showSearch for |
What do both rho dependent and rho independent mechanisms of termination have in common?
What is the key similarity and difference between ρ dependent and ρ independent transcription termination? In both cases, a stem loop structure causes RNA polymerase to pause. In ρ dependent termination, ρ protein is required to interact with the open complex at this point, dissociating the RNA-DNA hybrid.
How does rho dependent termination occur in prokaryotes?
Rho-dependent termination is caused by the rho protein colliding with the stalled polymerase at a stretch of G nucleotides on the DNA template near the end of the gene.
How does rho-dependent termination occur in prokaryotes?
Is rho-dependent termination only in prokaryotes?
What is the termination sequence for transcription?
In genetics, a transcription terminator is a section of nucleic acid sequence that marks the end of a gene or operon in genomic DNA during transcription. This sequence mediates transcriptional termination by providing signals in the newly synthesized transcript RNA that trigger processes which release…
What is intrinsic termination?
Intrinsic termination (also called Rho -independent termination) is a mechanism in both eukaryotes and prokaryotes that causes mRNA transcription to be stopped. In this mechanism, the mRNA contains a sequence that can base pair with itself to form a stem-loop structure 7-20 base pairs in length that is also rich in Cytosine – Guanine base pairs.
What is the termination of transcription?
Termination. Termination of transcription is triggered when the RNA polymerase encounters a particular DNA sequence, causing the polymerase to lose affinity for the DNA template. At this point, RNA polymerase disengages from the DNA and the RNA molecule is released for translation or post-transcriptional processing.
What is termination signal in biology?
Termination signal. A termination signal is found at the end of the part of the chromosome being transcribed during transcription of mRNA.