What is the difference between pour plate and spread plate method?
The key difference between Pour plate and Spread plate is that a known volume of the sample is spread on the surface of the agar medium in spread plate, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate.
What is the advantage of spread plate over pour plate?
Heat sensitive microbes are not affected. No subsurface colonies appear in spread plate so isolation of the organism is easy.
What is pour plating method?
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate. Each colony represents a “colony-forming unit” (CFU).
What is the spread plate method used for?
The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate.
Why is pour plate more accurate?
Advantages of Pour Plate Technique Will detect lower concentrations than surface spread method because of the larger sample volume. It requires no pre-drying of the agar surface. The most common method for determining the total viable count is the pour-plate method.
What are the basic differences between the streak plate and the pour plate methods of isolation?
The main difference between streak plate and pour plate is that in streak plate, the first to be added is the melted nutrient agar and the second to be added is a loop of bacteria from a slant, whereas the first to be added in pour plate is the bacterial broth and the second to be added is the nutrient agar.
What are the advantages and disadvantages of spread plate method?
Advantages and Disadvantages of Spread Plate Technique
- The optimum method for aerobes while microaerophilic tends to grow slower.
- The dilutions should be Accurate.
- The volume of inoculum greater than 0.1 mL on the nutrient agar not soak well and may result in colonies to coalesce as they form.
What is pour plate and spread plate?
Definition. Pour Plate: A plate prepared by mixing the inoculum with the cooled but still molten medium before pouring the latter into the Petri dish. Spread Plate: A technique used to count or isolate bacterial colonies on the surface of the agar.
What is the main advantage of the pour plate method?
What are the main advantages of pour plate technique?
What are the advantages of pour plate method?
Advantages of Pour Plate Technique Easy to undertake. Will detect lower concentrations than surface spread method because of the larger sample volume. It requires no pre-drying of the agar surface. The most common method for determining the total viable count is the pour-plate method.
What’s the difference between a spread plate and a pour plate?
Pour plate technique is a microbial method to enumerate some viable cells present in a sample. The speciality of the pour plate method is that a known volume of the sample is first mixed with agar and then poured into the plate. Other steps are similar to the spread plate technique discussed in the next section.
Which is the speciality of the pour plate method?
Pour plate technique is a microbial method to enumerate some viable cells present in a sample. The speciality of the pour plate method is that a known volume of the sample is first mixed with agar and then poured into the plate.
How much agar is used in the pour plate method?
In this method, fixed amount of inoculum (generally 1 ml) from a broth/sample is placed in the center of sterile Petri dish using a sterile pipette. Molten cooled agar (approx. 15mL) is then poured into the Petri dish containing the inoculum and mixed well.
Why are pour plates used in colony counting?
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plat e.