Why is selective precipitation important?
Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step.
What is precipitation in analytical chemistry?
In aqueous solution, precipitation is the process of transforming a dissolved substance into an insoluble solid from a super-saturated solution. In case of an inorganic chemical reaction leading to precipitation, the chemical reagent causing the solid to form is called the precipitant.
What is the method of precipitation?
Precipitation is a technique used to separate a mixture based on the solubility of its components. A dissolved compound can be precipitated out of solution by introducing a counter ion. For example, silver can be precipitated out of solution in the reaction between silver nitrate and sodium chloride.
What is the purpose of precipitation?
Precipitation often is used to remove metal ions from aqueous solutions: silver ions present in a solution of a soluble salt, such as silver nitrate, are precipitated by addition of chloride ions, provided, for example, by a solution of sodium chloride; the chloride ions and the silver ions combine to form silver …
What is selective precipitation?
Selective precipitation is a technique of separating ions in an aqueous solution by using a reagent that precipitates one or more of the ions, while leaving other ions in solution. Qualitative Analysis for Metallic Elements.
What is used for selective precipitation of proteins?
Ammonium sulfate is the best, first-choice salt for initial development of a salting-out program to precipitate sought-for proteins (see Fig. 4.5. 2).
What is selective solubility?
[si′lek·tiv ‚säl·yə′bil·əd·ē di‚fyü·zhən] (chemical engineering) The transmission of fluids through a nonporous, polymeric barrier (membrane) by an adsorption-solution-diffusion-desorption sequence.
What is selective precipitation in chemistry?
Which acid is used for selective precipitation of proline?
With the aid of the rhodanilate method a determination of proline in gelatin was performed. From a hydrochloric acid hydrolysate of gelatin, arginine was first removed by flavianic acid. Then ammonium rhodanilate was added to the mother liquor as long as a uniform precipitation of the proline salt took place.